DNA glycosylases in the base excision repair of DNA

HE Krokan, R Standal, G Slupphaug - Biochemical Journal, 1997 - portlandpress.com
A wide range of cytotoxic and mutagenic DNA bases are removed by different DNA
glycosylases, which initiate the base excision repair pathway. DNA glycosylases cleave the …

A high-coverage Neandertal genome from Vindija Cave in Croatia

K Prüfer, C De Filippo, S Grote, F Mafessoni, P Korlević… - Science, 2017 - science.org
To date, the only Neandertal genome that has been sequenced to high quality is from an
individual found in Southern Siberia. We sequenced the genome of a female Neandertal …

Manual and automated preparation of single-stranded DNA libraries for the sequencing of DNA from ancient biological remains and other sources of highly degraded …

MT Gansauge, A Aximu-Petri, S Nagel, M Meyer - Nature Protocols, 2020 - nature.com
It has been shown that highly fragmented DNA is most efficiently converted into DNA
libraries for sequencing if both strands of the DNA fragments are processed independently …

A high-coverage genome sequence from an archaic Denisovan individual

M Meyer, M Kircher, MT Gansauge, H Li, F Racimo… - Science, 2012 - science.org
We present a DNA library preparation method that has allowed us to reconstruct a high-
coverage (30×) genome sequence of a Denisovan, an extinct relative of Neandertals. The …

Partial uracil–DNA–glycosylase treatment for screening of ancient DNA

N Rohland, E Harney, S Mallick… - … Transactions of the …, 2015 - royalsocietypublishing.org
The challenge of sequencing ancient DNA has led to the development of specialized
laboratory protocols that have focused on reducing contamination and maximizing the …

Ancient genomic changes associated with domestication of the horse

P Librado, C Gamba, C Gaunitz, C Der Sarkissian… - Science, 2017 - science.org
The genomic changes underlying both early and late stages of horse domestication remain
largely unknown. We examined the genomes of 14 early domestic horses from the Bronze …

A nucleotide-flip** mechanism from the structure of human uracil–DNA glycosylase bound to DNA

G Slupphaug, CD Mol, B Kavli, AS Arvai, HE Krokan… - Nature, 1996 - nature.com
ANY uracil bases in DNA, a result of either misincorporation or deamination of cytosine, are
removed by uracil-DNA glycosylase (UDG), one of the most efficient and specific of the base …

[HTML][HTML] Base excision repair initiation revealed by crystal structures and binding kinetics of human uracil-DNA glycosylase with DNA

SS Parikh, CD Mol, G Slupphaug, S Bharati… - The EMBO …, 1998 - embopress.org
Three high-resolution crystal structures of DNA complexes with wild-type and mutant human
uracil-DNA glycosylase (UDG), coupled kinetic characterizations and comparisons with the …

Comparing the performance of three ancient DNA extraction methods for high‐throughput sequencing

C Gamba, K Hanghøj, C Gaunitz… - Molecular Ecology …, 2016 - Wiley Online Library
The DNA molecules that can be extracted from archaeological and palaeontological
remains are often degraded and massively contaminated with environmental microbial …

Uracil-excision DNA repair

DW Mosbaugh, SE Bennett - Progress in nucleic acid research and …, 1994 - Elsevier
Publisher Summary Uracil residues are introduced into prokaryotic and eukaryotic
deoxyribonucleic acid (DNA) as a normal physiological process during DNA synthesis, and …