Protein-protein interactions are fundamental to the formation of intricate interaction networks
and the assembly of multisubunit protein complexes that represent the functional …

Highlights•CX-MS identifies protein–protein interactions.•Cross-linking strategies targeting
different amino acids are available.•Combining CX-MS and cryo-EM delivers high-resolution …

Introduction: Protein cross-links are common in biological systems and are generated both
deliberately as a part of normal metabolism and also accidently as a result of exposure to …

Protein chemical cross-linking combined with mass spectrometry has become an important
technique for the analysis of protein structure and protein–protein interactions. A variety of …

Protein cross-links are formed in regulated biochemical processes in many biological
systems, but they are also generated inadvertently via the reactions of exogenous or …

We have investigated the molecular-level structure of the Vaccinia virion in situ by protein-
protein chemical crosslinking, identifying 4609 unique-mass crosslink ions at an effective …

Chemical cross‐linking analyzed by mass spectrometry (XL‐MS) has become an important
tool in unravelling protein structure, dynamics, and complex formation. Because the analysis …

During the last two decades, cross-linking combined with mass spectrometry (MS) has
evolved as a valuable tool to gain structural insights into proteins and protein assemblies …

Background Cross-linking mass spectrometry (XL-MS) is a powerful technique for detecting
protein–protein interactions (PPIs) and modeling protein structures in a high-throughput …

Chemical cross-linking coupled to mass spectrometry is a powerful tool to study protein–
protein interactions and protein conformations. Two linked peptides are ionized and …