Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Time-correlated single photon counting (TCSPC) is based on the detection of single photons
of a periodic light signal, measurement of the detection time of the photons, and the build-up …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Metabolic imaging of the relative amounts of reduced NADH and FAD and the
microenvironment of these metabolic electron carriers can be used to noninvasively monitor …

Imaging techniques based on retinal autofluorescence have found broad applications in
ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus …

Fundus autofluorescence (FAF) imaging is a novel imaging method that allows topographic
map** of lipofuscin distribution in the retinal pigment epithelium cell monolayer as well as …

This article presents an overview of time-resolved (lifetime) fluorescence techniques used in
biomedical diagnostics. In particular, we review the development of time-resolved …

Functional alterations are first signs of a starting pathological process. A device that
measures parameter for the characterization of the metabolism at the human eye‐ground …

The study of biological systems in their real environmental conditions is crucial to decipher
the true image of structures and processes underlying their functionality. In this regard …