Agrobacterium-mediated sorghum transformation frequency has been enhanced
significantly via medium optimization using immature embryos from sorghum variety TX430 …

Biotechnology provides a means for the rapid genetic improvement of plants. Although
single genes have been important in engineering herbicide and pest tolerance traits in …

Recombineering, permitting precise modification of genes within bacterial artificial
chromosomes (BACs) through homologous recombination mediated by lambda phage …

One of the most information‐rich aspects of gene functional studies is characterization of
gene expression profiles at cellular resolution, and subcellular localization of the …

Bacterial artificial chromosome (BAC) libraries are the large DNA insert libraries of choice
and valuable tools for the map-based cloning of target quantitative trait loci, physical …

Bacterial artificial chromosomes (BACs) are widely used for the construction of physical
maps, positional-cloning and whole-genome sequencing strategies. Unfortunately, their use …

Pulse crops are considered minor on a global scale despite their nutritional value for human
consumption. Therefore, they are relatively less extensively studied in comparison with the …

Advancement in plant research is becoming impaired by the fact that the transfer of multiple
genes is difficult to achieve. Here we present a new binary vector for Agrobacterium …

The ability to stack multiple genes in plants is of great importance in the development of
crops with desirable traits but can be challenging due to limited selectable marker options …

Background Large-insert BAC and BIBAC libraries are important tools for structural and
functional genomics studies of eukaryotic genomes. To facilitate the construction of BAC and …