Real-time monitoring of phosphodiesterase inhibition in intact cells
Phosphodiesterases (PDEs) are hydrolytic enzymes, which convert cyclic AMP (cAMP) and
cyclic GMP (cGMP) into their corresponding monophosphates. PDE-dependent hydrolysis …
cyclic GMP (cGMP) into their corresponding monophosphates. PDE-dependent hydrolysis …
A novel PDE2A reporter cell line: characterization of the cellular activity of PDE inhibitors
F Wunder, MJ Gnoth, A Geerts… - Molecular …, 2009 - ACS Publications
We report here the generation and pharmacological characterization of a
phosphodiesterase 2A (PDE2A) reporter cell line. Human PDE2A was stably transfected in a …
phosphodiesterase 2A (PDE2A) reporter cell line. Human PDE2A was stably transfected in a …
A cyclic nucleotide sensitive promoter reporter system suitable for bacteria and plant cells
Abstract Background Cyclic AMP (cAMP) and cyclic GMP (cGMP) have roles in relaying
external signals and modifying gene expression within cells in all phyla. Currently there are …
external signals and modifying gene expression within cells in all phyla. Currently there are …
A cell-based PDE4 assay in 1536-well plate format for high-throughput screening
The cyclic nucleotide phosphodiesterases (PDEs) are intracellular enzymes that catalyze
the hydrolysis of 3,′ 5′-cyclic nucleotides, such as cyclic adenosine monophosphate …
the hydrolysis of 3,′ 5′-cyclic nucleotides, such as cyclic adenosine monophosphate …
Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors
Phosphodiesterases (PDEs) constitute a superfamily of enzymes that plays an important role
in signal transduction by catalysing the hydrolysis of cAMP and cGMP. cDNA encoding …
in signal transduction by catalysing the hydrolysis of cAMP and cGMP. cDNA encoding …
Characterization of the cellular activity of PDE 4 inhibitors using two novel PDE 4 reporter cell lines
F Wunder, R Quednau, A Geerts, M Barg… - Molecular …, 2013 - ACS Publications
We report here the generation and pharmacological characterization of two novel PDE 4B1
and PDE 4D3 reporter cell lines. Intracellular cAMP levels are monitored in these cells by a …
and PDE 4D3 reporter cell lines. Intracellular cAMP levels are monitored in these cells by a …
Development of a cell‐based assay for screening of phosphodiesterase 10A (PDE10A) inhibitors using a stable recombinant HEK‐293 cell line expressing high levels …
RS Bora, D Gupta, R Malik, S Chachra… - Biotechnology and …, 2008 - Wiley Online Library
cDNA encoding PDE10A (phosphodiesterase 10A) was cloned and a stable recombinant
HEK‐293 (human embryonic kidney‐293) cell line expressing high levels of PDE10A was …
HEK‐293 (human embryonic kidney‐293) cell line expressing high levels of PDE10A was …
Development of a high-throughput assay for monitoring cAMP levels in cardiac ventricular myocytes
KB Walsh, TC Rich, ZJ Coffman - Journal of Cardiovascular …, 2009 - journals.lww.com
G-protein-coupled receptors (GPCRs) represent the largest family of transmembrane
receptors involved in cell signal transduction. Many of these GPCRs convey their …
receptors involved in cell signal transduction. Many of these GPCRs convey their …
Production and characterization of pharmacologically active recombinant human phosphodiesterase 4B in Dictyostelium discoideum
Abstract Phosphodiesterase 4B (PDE4B) is an important therapeutic target for asthma and
chronic obstructive pulmonary disease. To identify PDE4 subtype‐specific compounds using …
chronic obstructive pulmonary disease. To identify PDE4 subtype‐specific compounds using …
Optimization and validation of a reporter gene assay for screening of phosphodiesterase inhibitors in a high throughput system
Phosphodiesterases (PDEs) hydrolyze cyclic nucleotides, cyclic adenosine monophosphate
(cAMP) and guanosine monophosphate (cGMP) into inactive 5'monophosphates, and exist …
(cAMP) and guanosine monophosphate (cGMP) into inactive 5'monophosphates, and exist …