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Mass-spectrometric exploration of proteome structure and function
Numerous biological processes are concurrently and coordinately active in every living cell.
Each of them encompasses synthetic, catalytic and regulatory functions that are, almost …
Each of them encompasses synthetic, catalytic and regulatory functions that are, almost …
Challenges and emergent solutions for LC‐MS/MS based untargeted metabolomics in diseases
In the past decade, advances in liquid chromatography‐mass spectrometry (LC‐MS) have
revolutionized untargeted metabolomics analyses. By mining metabolomes more deeply …
revolutionized untargeted metabolomics analyses. By mining metabolomes more deeply …
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition
Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes
in a fast and reproducible manner. Here we present the narrow-window data-independent …
in a fast and reproducible manner. Here we present the narrow-window data-independent …
Global detection of human variants and isoforms by deep proteome sequencing
An average shotgun proteomics experiment detects approximately 10,000 human proteins
from a single sample. However, individual proteins are typically identified by peptide …
from a single sample. However, individual proteins are typically identified by peptide …
[HTML][HTML] What are the 100 most cited fungal genera?
The global diversity of fungi has been estimated between 2 to 11 million species, of which
only about 155 000 have been named. Most fungi are invisible to the unaided eye, but they …
only about 155 000 have been named. Most fungi are invisible to the unaided eye, but they …
[HTML][HTML] Optimization of experimental parameters in data-independent mass spectrometry significantly increases depth and reproducibility of results
Comprehensive, reproducible and precise analysis of large sample cohorts is one of the key
objectives of quantitative proteomics. Here, we present an implementation of data …
objectives of quantitative proteomics. Here, we present an implementation of data …
De novo peptide sequencing by deep learning
De novo peptide sequencing from tandem MS data is the key technology in proteomics for
the characterization of proteins, especially for new sequences, such as mAbs. In this study …
the characterization of proteins, especially for new sequences, such as mAbs. In this study …
The Perseus computational platform for comprehensive analysis of (prote) omics data
A main bottleneck in proteomics is the downstream biological analysis of highly multivariate
quantitative protein abundance data generated using mass-spectrometry-based analysis …
quantitative protein abundance data generated using mass-spectrometry-based analysis …
Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry
Quantitative proteomics employing mass spectrometry is an indispensable tool in life
science research. Targeted proteomics has emerged as a powerful approach for …
science research. Targeted proteomics has emerged as a powerful approach for …
Ultrasensitive proteome analysis using paramagnetic bead technology
In order to obtain a systems‐level understanding of a complex biological system, detailed
proteome information is essential. Despite great progress in proteomics technologies …
proteome information is essential. Despite great progress in proteomics technologies …