Nano-LC in proteomics: recent advances and approaches

SR Wilson, T Vehus, HS Berg, E Lundanes - Bioanalysis, 2015‏ - Taylor & Francis
In proteomics, nano-LC is arguably the most common tool for separating peptides/proteins
prior to MS. The main advantage of nano-LC is enhanced sensitivity, as compounds enter …

High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation

S Shen, X Wang, X Zhu, S Rasam, M Ma, S Huo… - Nature protocols, 2023‏ - nature.com
Robust, reliable quantification of large sample cohorts is often essential for meaningful
clinical or pharmaceutical proteomics investigations, but it is technically challenging. When …

IonStar enables high-precision, low-missing-data proteomics quantification in large biological cohorts

X Shen, S Shen, J Li, Q Hu, L Nie, C Tu, X Wang… - Proceedings of the …, 2018‏ - pnas.org
Reproducible quantification of large biological cohorts is critical for clinical/pharmaceutical
proteomics yet remains challenging because most prevalent methods suffer from drastically …

An IonStar experimental strategy for MS1 ion current-based quantification using ultrahigh-field orbitrap: reproducible, in-depth, and accurate protein measurement in …

X Shen, S Shen, J Li, Q Hu, L Nie, C Tu… - Journal of proteome …, 2017‏ - ACS Publications
In-depth and reproducible protein measurement in many biological samples is often critical
for pharmaceutical/biomedical proteomics but remains challenging. MS1-based …

MS1 ion current‐based quantitative proteomics: A promising solution for reliable analysis of large biological cohorts

X Wang, S Shen, SS Rasam, J Qu - Mass spectrometry reviews, 2019‏ - Wiley Online Library
The rapidly‐advancing field of pharmaceutical and clinical research calls for systematic,
molecular‐level characterization of complex biological systems. To this end, quantitative …

Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry

BL Parker, G Yang, SJ Humphrey, R Chaudhuri… - Science …, 2015‏ - science.org
A major goal in signaling biology is the establishment of high-throughput quantitative
methods for measuring changes in protein phosphorylation of entire signal transduction …

A novel PDE4D inhibitor BPN14770 reverses scopolamine-induced cognitive deficits via cAMP/SIRT1/Akt/Bcl-2 pathway

Y Wang, S Gao, V Zheng, L Chen, M Ma… - Frontiers in cell and …, 2020‏ - frontiersin.org
A global, quantitative proteomics/systems-biology analysis of the selective pharmacological
inhibition of phosphodiesterase-4D (PDE4D) revealed the differential regulation of pathways …

Surfactant-aided precipitation/on-pellet-digestion (SOD) procedure provides robust and rapid sample preparation for reproducible, accurate and sensitive LC/MS …

B An, M Zhang, RW Johnson, J Qu - Analytical chemistry, 2015‏ - ACS Publications
For targeted protein quantification by liquid chromatography mass spectrometry (LC/MS), an
optimal approach for efficient, robust and hi-throughput sample preparation is critical, but …

Surfactant cocktail-aided extraction/precipitation/on-pellet digestion strategy enables efficient and reproducible sample preparation for large-scale quantitative …

S Shen, B An, X Wang, SP Hilchey, J Li, J Cao… - Analytical …, 2018‏ - ACS Publications
For quantitative proteomics, efficient, robust, and reproducible sample preparation with high
throughput is critical yet challenging, especially when large cohorts are involved, as is often …

Transitioning from basic toward systems pharmacodynamic models: lessons from corticosteroids

VS Ayyar, WJ Jusko - Pharmacological Reviews, 2020‏ - Elsevier
Technology in bioanalysis,-omics, and computation have evolved over the past half century
to allow for comprehensive assessments of the molecular to whole body pharmacology of …