Extreme environments offer an unprecedented opportunity to understand microbial eukaryotic ecology, evolution, and genome biology

HB Rappaport, AM Oliverio - Nature Communications, 2023 - nature.com
Research in extreme environments has substantially expanded our understanding of the
ecology and evolution of life on Earth, but a major group of organisms has been largely …

[HTML][HTML] Computational methods for 16S metabarcoding studies using Nanopore sequencing data

A Santos, R van Aerle, L Barrientos… - Computational and …, 2020 - Elsevier
Assessment of bacterial diversity through sequencing of 16S ribosomal RNA (16S rRNA)
genes has been an approach widely used in environmental microbiology, particularly since …

Comprehensive benchmarking and ensemble approaches for metagenomic classifiers

ABR McIntyre, R Ounit, E Afshinnekoo, RJ Prill… - Genome biology, 2017 - Springer
Background One of the main challenges in metagenomics is the identification of
microorganisms in clinical and environmental samples. While an extensive and …

Making and sequencing heavily multiplexed, high-throughput 16S ribosomal RNA gene amplicon libraries using a flexible, two-stage PCR protocol

A Naqib, S Poggi, W Wang, M Hyde… - … analysis: Methods and …, 2018 - Springer
Deep sequencing of polymerase chain reaction (PCR)-amplified small subunit (16S or 18S)
ribosomal RNA (rRNA) genes fragments is commonly employed to characterize the …

Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect …

M Gand, B Bloemen, K Vanneste, NHC Roosens… - BMC genomics, 2023 - Springer
Abstract Background Oxford Nanopore Technologies (ONT) offers an accessible platform for
long-read sequencing, which improves the reconstruction of genomes and helps to resolve …

Metagenomic assembly through the lens of validation: recent advances in assessing and improving the quality of genomes assembled from metagenomes

ND Olson, TJ Treangen, CM Hill… - Briefings in …, 2019 - academic.oup.com
Metagenomic samples are snapshots of complex ecosystems at work. They comprise
hundreds of known and unknown species, contain multiple strain variants and vary greatly …

[HTML][HTML] Loop-mediated isothermal amplification detection of SARS-CoV-2 and myriad other applications

KJM Moore, J Cahill, G Aidelberg… - Journal of …, 2021 - ncbi.nlm.nih.gov
As the second year of the COVID-19 pandemic begins, it remains clear that a massive
increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to …

The application of metagenomics to study microbial communities and develop desirable traits in fermented foods

M Srinivas, O O'Sullivan, PD Cotter, D Sinderen… - Foods, 2022 - mdpi.com
The microbial communities present within fermented foods are diverse and dynamic,
producing a variety of metabolites responsible for the fermentation processes, imparting …

Metagenomic analysis of geothermal water reservoir sites exploring carbohydrate-related thermozymes

G Kaushal, J Kumar, RS Sangwan, SP Singh - International journal of …, 2018 - Elsevier
Hot springs are geothermally heated underground water that create a natural habitat for
diverse thermophilic microorganisms. The study presents a comprehensive investigation of …

Finding a needle in a haystack: Bacteroides fragilis polysaccharide A as the archetypical symbiosis factor

D Erturk‐Hasdemir, DL Kasper - … of the New York Academy of …, 2018 - Wiley Online Library
Starting from birth, all animals develop a symbiotic relationship with their resident
microorganisms that benefits both the microbe and the host. Recent advances in technology …