Covering: 2000 up to 2018 The cytochromes P450 (P450s) are a superfamily of heme-
containing monooxygenases that perform diverse catalytic roles in many species, including …

Highlights•Cytochrome P450 enzymes catalyse oxygen insertion into a wide range of
substrates.•Mutagenesis of P450s frequently allows novel substrate recognition.•P450s …

A grand challenge in the field of structural biology is to design and engineer proteins that
exhibit targeted functions. Although much success on this front has been achieved, design …

Covering: up to January 2017 Cytochrome P450 enzymes (P450s) are some of the most
exquisite and versatile biocatalysts found in nature. In addition to their well-known roles in …

Cytochrome P450s (Cyt P450s) and peroxidases are enzymes featuring iron heme cofactors
that have wide applicability as biocatalysts in chemical syntheses. Cyt P450s are a family of …

Fusion proteins, understood as those created by joining two or more genes that originally
encoded independent proteins, have numerous applications in biotechnology, from …

Natural products that contain functional groups with heteroatom-heteroatom linkages (X–X,
where X= N, O, S, and P) are a small yet intriguing group of metabolites. The reactivity and …

Tryptophan, the most chemically complex and the least abundant of the 20 common
proteinogenic amino acids, is a biosynthetic precursor to a large number of complex …

One of the greatest challenges in protein design is creating new enzymes, something
evolution does all the time, starting from existing ones. Borrowing from nature's evolutionary …

We recently demonstrated that variants of cytochrome P450BM3 (CYP102A1) catalyze the
insertion of nitrogen species into benzylic C–H bonds to form new C–N bonds. An …