Achieving cost-competitive bio-based processes requires development of stable and
selective biocatalysts. Their realization through in vitro enzyme characterization and …

The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

Microalgae are regarded as promising organisms to develop innovative concepts based on
their photosynthetic capacity that offers more sustainable production than heterotrophic …

The development of more sustainable food, feed, and bio-products is critical to mitigating the
environmental stresses facing our world today. Algae, which includes seaweeds, eukaryotic …

DNA-amplicon-based microbiota profiling can estimate species diversity and abundance but
cannot resolve genetic differences within individuals of the same species. Here we report …

In the first wave of synthetic biology, genetic elements, combined into simple circuits, are
used to control individual cellular functions. In the second wave of synthetic biology, the …

Synthetic biology relies on the manufacture of large and complex DNA constructs from
libraries of genetic parts. Golden Gate and other Type IIS restriction enzyme-dependent …

Large-scale data acquisition and analysis are often required in the successful
implementation of the design, build, test, and learn (DBTL) cycle in biosystems design …

Competitive sustainable production in industry demands new and better biocatalysts,
optimized bioprocesses and cost-effective product recovery. Our review sheds light on the …

As redesigning organisms using engineering principles is one of the purposes of synthetic
biology (SynBio), the standardization of experimental methods and DNA parts is becoming …