Metabolomics by numbers: acquiring and understanding global metabolite data

R Goodacre, S Vaidyanathan, WB Dunn… - TRENDS in …, 2004‏ - cell.com
In this postgenomic era, there is a specific need to assign function to orphan genes in order
to validate potential targets for drug therapy and to discover new biomarkers of disease …

Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses

HM Davey, DB Kell - Microbiological reviews, 1996‏ - journals.asm.org
The most fundamental questions such as whether a cell is alive, in the sense of being able
to divide or to form a colony, may sometimes be very hard to answer, since even axenic …

How fast‐growing bacteria robustly tune their ribosome concentration to approximate growth‐rate maximization

E Bosdriesz, D Molenaar, B Teusink… - The FEBS …, 2015‏ - Wiley Online Library
Maximization of growth rate is an important fitness strategy for bacteria. Bacteria can achieve
this by expressing proteins at optimal concentrations, such that resources are not wasted …

Quantitative nature of overexpression experiments

H Moriya - Molecular biology of the cell, 2015‏ - molbiolcell.org
Overexpression experiments are sometimes considered as qualitative experiments
designed to identify novel proteins and study their function. However, in order to draw …

Why is metabolic labour divided in nitrification?

E Costa, J Pérez, JU Kreft - Trends in microbiology, 2006‏ - cell.com
Winogradsky discovered in 1890 that nitrification is carried out in two consecutive steps by
two distinct groups of bacteria: ammonia-oxidizing bacteria and nitrite-oxidizing bacteria. An …

The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters

PR Jensen, K Hammer - Applied and environmental microbiology, 1998‏ - journals.asm.org
We constructed a library of synthetic promoters for Lactococcus lactis in which the known
consensus sequences were kept constant while the sequences of the separating spacers …

Auxotrophic yeast strains in fundamental and applied research

JT Pronk - Applied and environmental microbiology, 2002‏ - journals.asm.org
Molecular genetic tools have become increasingly important in fundamental and applied
yeast research. Genetic modifications, such as the targeted inactivation of genes or their …

Anaerobic Xylose Fermentation by Recombinant Saccharomyces cerevisiae Carrying XYL1, XYL2, andXKS1 in Mineral Medium Chemostat Cultures

A Eliasson, C Christensson, CF Wahlbom… - Applied and …, 2000‏ - journals.asm.org
For ethanol production from lignocellulose, the fermentation of xylose is an economic
necessity. Saccharomyces cerevisiae has been metabolically engineered with a xylose …

Role of cultivation media in the development of yeast strains for large scale industrial use

B Hahn-Hägerdal, K Karhumaa, CU Larsson… - Microbial cell …, 2005‏ - Springer
The composition of cultivation media in relation to strain development for industrial
application is reviewed. Heterologous protein production and pentose utilization by …

Searching for principles of microbial physiology

FJ Bruggeman, R Planqué, D Molenaar… - FEMS microbiology …, 2020‏ - academic.oup.com
Why do evolutionarily distinct microorganisms display similar physiological behaviours?
Why are transitions from high-ATP yield to low (er)-ATP yield metabolisms so widespread …