Genome integrity is constantly monitored by sophisticated cellular networks, collectively
termed the DNA damage response (DDR). A common feature of DDR proteins is their …

Genomes are transmitted faithfully from dividing cells to their offspring. Changes that occur
during DNA repair, chromosome duplication, and transmission or via recombination provide …

Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along
the genome is instrumental to understanding genomic fragility, but current methods are …

We describe a Hi-C-based method, Micro-C, in which micrococcal nuclease is used instead
of restriction enzymes to fragment chromatin, enabling nucleosome resolution chromosome …

We present a genome-wide approach to map DNA double-strand breaks (DSBs) at
nucleotide resolution by a method we termed BLESS (direct in situ breaks labeling …

Completion of genome duplication is challenged by structural and topological barriers that
impede progression of replication forks. Although this can seriously undermine genome …

Transcriptional R loops are anomalous RNA: DNA hybrids that have been detected in
organisms from bacteria to humans. These structures have been shown in eukaryotes to …

The mechanisms that maintain the stability of chromosome ends have broad impact on
genome integrity in all eukaryotes. Budding yeast is a premier organism for telomere …

Covalent histone modifications are highly conserved and play multiple roles in eukaryotic
transcription regulation. Here, we mapped 26 histone modifications genome-wide in …

As genome-editing nucleases move toward broader clinical applications, the need to define
the limits of their specificity and efficiency increases. A variety of approaches for nuclease …