[HTML][HTML] Apoptosis: A mammalian cell bioprocessing perspective
Apoptosis is a form of programmed and controlled cell death that accounts for the majority of
cellular death in bioprocesses. Cell death affects culture longevity and product quality; it is …
cellular death in bioprocesses. Cell death affects culture longevity and product quality; it is …
In vivo oxygen, temperature and pH dynamics in the female reproductive tract and their importance in human conception: a systematic review
BACKGROUND Despite advances in ART, implantation and pregnancy rates per embryo
transfer still remain low. IVF laboratories strive to ensure that the process of handling …
transfer still remain low. IVF laboratories strive to ensure that the process of handling …
Dynamic culture of mesenchymal stromal/stem cell spheroids and secretion of paracrine factors
P Fuentes, MJ Torres, R Arancibia… - … in Bioengineering and …, 2022 - frontiersin.org
In recent years, conditioned medium (CM) obtained from the culture of mesenchymal
stromal/stem cells (MSCs) has been shown to effectively promote tissue repair and modulate …
stromal/stem cells (MSCs) has been shown to effectively promote tissue repair and modulate …
[HTML][HTML] Mammalian systems biotechnology reveals global cellular adaptations in a recombinant CHO cell line
Effective development of host cells for therapeutic protein production is hampered by the
poor characterization of cellular transfection. Here, we employed a multi-omics-based …
poor characterization of cellular transfection. Here, we employed a multi-omics-based …
Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production
Improving the cellular capacity of Chinese hamster ovary (CHO) cells to produce large
amounts of therapeutic proteins remains a major challenge for the biopharmaceutical …
amounts of therapeutic proteins remains a major challenge for the biopharmaceutical …
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions
Recombinant adeno‐associated viral vectors (rAAVs) are one of the most used vehicles for
gene therapy, with five rAAV therapeutics commercially approved by the FDA. To improve …
gene therapy, with five rAAV therapeutics commercially approved by the FDA. To improve …
Revealing key determinants of clonal variation in transgene expression in recombinant CHO cells using targeted genome editing
Generation of recombinant Chinese hamster ovary (rCHO) cell lines is critical for the
production of therapeutic proteins. However, the high degree of phenotypic heterogeneity …
production of therapeutic proteins. However, the high degree of phenotypic heterogeneity …
Systematic development of temperature shift strategies for Chinese hamster ovary cells based on short duration cultures and kinetic modeling
Temperature shift (TS) to a hypothermic condition has been widely used during protein
production processes that use Chinese hamster ovary (CHO) cells. The effect of temperature …
production processes that use Chinese hamster ovary (CHO) cells. The effect of temperature …
Temperature down‐shift modifies expression of UPR‐/ERAD‐related genes and enhances production of a chimeric fusion protein in CHO cells
M Torres, S Akhtar, EA McKenzie… - Biotechnology …, 2021 - Wiley Online Library
Low culture temperature enhances the cell‐specific productivity of Chinese hamster ovary
(CHO) cells expressing varied recombinant (r‐) proteins, but the mechanisms remain …
(CHO) cells expressing varied recombinant (r‐) proteins, but the mechanisms remain …
Effective temperature shift strategy development and scale confirmation for simultaneous optimization of protein productivity and quality in Chinese hamster ovary cells
KP McHugh, J Xu, KL Aron, MC Borys… - Biotechnology …, 2020 - Wiley Online Library
Temperature shifts to lower culture temperatures are frequently employed in the
manufacturing of protein therapeutics in mammalian cells to improve productivity, viability, or …
manufacturing of protein therapeutics in mammalian cells to improve productivity, viability, or …