Advanced technologies for the preservation of mammalian biospecimens

H Huang, X He, ML Yarmush - Nature biomedical engineering, 2021 - nature.com
The three classical core technologies for the preservation of live mammalian biospecimens—
slow freezing, vitrification and hypothermic storage—limit the biomedical applications of …

Grapevine shoot tip cryopreservation and cryotherapy: Secure storage of disease-free plants

JC Bettoni, Z Marković, W Bi, GM Volk, T Matsumoto… - Plants, 2021 - mdpi.com
Grapevine (Vitis spp.) is one of the most economically important temperate fruit crops.
Grapevine breeding programs require access to high-quality Vitis cultivars and wild species …

Cryopreservation of shoot tips of recalcitrant and tropical species: Advances and strategies

MN Normah, N Sulong, BM Reed - Cryobiology, 2019 - Elsevier
There is a pressing need for practical and successful conservation efforts to establish long-
term germplasm collections of recalcitrant and tropical species, given the challenge and …

Pre-treatment with salicylic acid improves plant regeneration after cryopreservation of grapevine (Vitis spp.) by droplet vitrification

R Pathirana, A McLachlan, D Hedderley… - Acta Physiologiae …, 2016 - Springer
Despite many reports on regeneration of Vitis after cryopreservation, there is no
cryopreserved collection of its germplasm. Some Vitis genotypes are reported to be …

[HTML][HTML] Cryotherapy: a new technique to obtain grapevine plants free of viruses

JC Bettoni, MD COSTA, JPP GARDIN… - Revista Brasileira de …, 2016 - SciELO Brasil
Through in vitro tissue culture techniques it is possible to propagate high quality nursery
plants faster. Cryotherapy is a promising tool, based on in vitro culture techniques, for …

Cryopreservation of grapevine (Vitis spp.)—a review

WL Bi, C Pan, XY Hao, ZH Cui, MM Kher… - In Vitro Cellular & …, 2017 - Springer
Grapevine (Vitis genus) is one of the economically most important fruits worldwide. Some
species and cultivars are rare and have only a few vines, but represent national heritages …

Development of an improved and simple shoot tip cryoconservation protocol for cryobanking of Bacopa monnieri (L.) Wettst. germplasm

R Gowthami, N Sharma, R Chandra, JS Kurian… - In Vitro Cellular & …, 2023 - Springer
Abstract Bacopa monnieri (L.) Wettst. is a high value medicinally important plant used as a
memory enhancer, the germplasm of which requires to be conserved long-term due to its …

Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro

JC Bettoni, AA Kretzschmar, R Bonnart, A Shepherd… - …, 2019 - journals.ashs.org
The availability of and easy access to diverse Vitis species are prerequisites for advances in
breeding programs. Plant genebanks usually maintain collections of Vitis taxa as field …

Droplet-vitrification cryopreservation of in vitro-grown shoot tips of grapevine (Vitis spp.)

WL Bi, XY Hao, ZH Cui, GM Volk, QC Wang - In Vitro Cellular & …, 2018 - Springer
An efficient and broad-spectrum protocol for cryopreservation of Vitis spp. shoot tips by
droplet-vitrification is reported. Shoot tips (1.0 mm) containing 5–6 leaf primordia (LPs) were …

Shoot tip cryotherapy for efficient eradication of grapevine leafroll‐associated virus‐3 from diseased grapevine in vitro plants

WL Bi, XY Hao, ZH Cui, R Pathirana… - Annals of Applied …, 2018 - Wiley Online Library
We describe a droplet‐vitrification cryotherapy method for the eradication of grapevine
leafroll‐associated virus‐3 (GLRaV‐3) from diseased in vitro shoots of Vitis plants. The …