Applications of immobilized lipases in enzymatic reactors: A review

D Remonatto, RH Miotti Jr, R Monti, JC Bassan… - Process …, 2022 - Elsevier
Lipases are efficient biocatalysts with numerous applications in different industrial sectors,
such as pharmaceutical, food, and fine chemistry industries. Enzyme immobilization further …

Efficient biodiesel production from waste cooking oil by fast co-immobilization of lipases from Aspergillus oryzae and Rhizomucor miehei in magnetic chitosan …

H Wei, Q Wang, R Zhang, M Liu, W Zhang - Process Biochemistry, 2023 - Elsevier
To achieve an efficient and cost-effective conversion from waste cooking oil (WCO) to
biodiesel, a facile one-pot co-immobilization of Aspergillus oryzae lipase (AOL) and …

What's new in flow biocatalysis? A snapshot of 2020–2022

M Crotti, MS Robescu, JM Bolivar, D Ubiali… - Frontiers in …, 2023 - frontiersin.org
Flow biocatalysis is a key enabling technology that is increasingly being applied to a wide
array of reactions with the aim of achieving process intensification, better control of …

Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design

M Tian, L Yang, P Lv, Z Wang, J Fu, C Miao, Z Li… - Bioresource …, 2022 - Elsevier
Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel
production. A semi-rational directed evolution method combined with N-glycosylation is …

Accelerated acidolysis for triglyceride modification using commercial lipases activated by a hydration–aggregation pretreatment

T Yoshioka, T Kuroiwa, K Wakahara, S Ozono - Process Biochemistry, 2023 - Elsevier
The modification of triglycerides (TGs) via acidolysis with fatty acids (FAs) was investigated
using three commercial lipases activated via hydration–aggregation pretreatment. Lipase …

Multiple strategies for high-efficiency expression of Thermomyces lanuginosus lipase in Pichia pastoris and production of biodiesel in solvent-free system

R Xu, Z Chen, Y Chen, X Wang, Y Zhang, X Li, F Wang - Fuel, 2023 - Elsevier
With the development of enzymatic routes toward biodiesel production, the process can be
concerned in a commercial viewpoint. The main bottleneck is the high cost of lipases. In this …

Enzymatic production process of capric acid-rich structured lipids: Development of formulation as a new therapeutic approach

RH Miotti Jr, SR do Amaral, AN Freitas… - International Journal of …, 2024 - Elsevier
The present work reports an optimization of the synthesis of MLM-type (medium, long,
medium) structured lipids (SL) through an acidolysis reaction of grape seed oil with capric …

Kinetic and thermodynamic study of enzymatic hydroesterification mechanism to fatty acid methyl esters synthesis

F Ketzer, JHC Wancura, MV Tres, JV de Oliveira - Bioresource Technology, 2022 - Elsevier
Eversa® Transform 2.0 lipase used as biocatalyst to biodiesel (fatty acid methyl esters–
FAME) synthesis has been the target of interesting studies due to its thermostability and cost …

Co-immobilization of lipases with different specificities for efficient and recyclable biodiesel production from waste oils: optimization using response surface …

Q Wang, R Zhang, M Liu, L Ma, W Zhang - International Journal of …, 2023 - mdpi.com
Lipase-catalyzed transesterification is a promising and sustainable approach to producing
biodiesel. To achieve highly efficient conversion of heterogeneous oils, combining the …

Tuning immobilized commercial lipase preparations features by simple treatment with metallic phosphate salts

JR Guimarães, D Carballares, PW Tardioli… - Molecules, 2022 - mdpi.com
Four commercial immobilized lipases biocatalysts have been submitted to modifications with
different metal (zinc, cobalt or copper) phosphates to check the effects of this modification on …