Macromolecular modeling and design in Rosetta: recent methods and frameworks

JK Leman, BD Weitzner, SM Lewis, J Adolf-Bryfogle… - Nature …, 2020 - nature.com
The Rosetta software for macromolecular modeling, docking and design is extensively used
in laboratories worldwide. During two decades of development by a community of …

Protein structure prediction from sequence variation

DS Marks, TA Hopf, C Sander - Nature biotechnology, 2012 - nature.com
Genomic sequences contain rich evolutionary information about functional constraints on
macromolecules such as proteins. This information can be efficiently mined to detect …

[HTML][HTML] High-resolution comparative modeling with RosettaCM

Y Song, F DiMaio, RYR Wang, D Kim, C Miles… - Structure, 2013 - cell.com
We describe an improved method for comparative modeling, RosettaCM, which optimizes a
physically realistic all-atom energy function over the conformational space defined by …

Protein 3D structure computed from evolutionary sequence variation

DS Marks, LJ Colwell, R Sheridan, TA Hopf, A Pagnani… - PloS one, 2011 - journals.plos.org
The evolutionary trajectory of a protein through sequence space is constrained by its
function. Collections of sequence homologs record the outcomes of millions of evolutionary …

Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes

T Thestrup, J Litzlbauer, I Bartholomäus, M Mues… - Nature …, 2014 - nature.com
The quality of genetically encoded calcium indicators (GECIs) has improved dramatically in
recent years, but high-performing ratiometric indicators are still rare. Here we describe a …

Determination of secondary structure populations in disordered states of proteins using nuclear magnetic resonance chemical shifts

C Camilloni, A De Simone, WF Vranken… - Biochemistry, 2012 - ACS Publications
One of the major open challenges in structural biology is to achieve effective descriptions of
disordered states of proteins. This problem is difficult because these states are …

Mitochondrial uncoupling protein 2 structure determined by NMR molecular fragment searching

MJ Berardi, WM Shih, SC Harrison, JJ Chou - Nature, 2011 - nature.com
Abstract Mitochondrial uncoupling protein 2 (UCP2) is an integral membrane protein in the
mitochondrial anion carrier protein family, the members of which facilitate the transport of …

Tau local structure shields an amyloid-forming motif and controls aggregation propensity

D Chen, KW Drombosky, Z Hou, L Sari… - Nature …, 2019 - nature.com
Tauopathies are neurodegenerative diseases characterized by intracellular amyloid
deposits of tau protein. Missense mutations in the tau gene (MAPT) correlate with …

Recent advances in spectroscopic techniques for the analysis of microplastics in food

X Guo, H Lin, S Xu, L He - Journal of Agricultural and Food …, 2022 - ACS Publications
Microplastic pollution has become a worldwide concern in aquatic and terrestrial
environments. Microplastics could also enter the food chain, causing potential harm to …

Miniaturization of NMR systems: Desktop spectrometers, microcoil spectroscopy, and “NMR on a chip” for chemistry, biochemistry, and industry

SS Zalesskiy, E Danieli, B Blumich… - Chemical …, 2014 - ACS Publications
Nuclear magnetic resonance spectroscopy (NMR) nowadays is one of the most powerful
and versatile analytical methods for modern organic chemistry,(1) catalysis,(2) biology,(3) …