Subcellular proteomics
The eukaryotic cell is compartmentalized into subcellular niches, including membrane-
bound and membrane-less organelles. Proteins localize to these niches to fulfil their …
bound and membrane-less organelles. Proteins localize to these niches to fulfil their …
Quantitative proteomic analysis of histone modifications
Several mechanisms have been proposed for histone marks to exert their functions,
including altering the physical properties of nucleosomes by neutralization of charge via …
including altering the physical properties of nucleosomes by neutralization of charge via …
Multi-omics analyses of the ulcerative colitis gut microbiome link Bacteroides vulgatus proteases with disease severity
Ulcerative colitis (UC) is driven by disruptions in host–microbiota homoeostasis, but current
treatments exclusively target host inflammatory pathways. To understand how host …
treatments exclusively target host inflammatory pathways. To understand how host …
Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry
Here we present an optimized workflow for global proteome and phosphoproteome analysis
of tissues or cell lines that uses isobaric tags (TMT (tandem mass tags)-10) for multiplexed …
of tissues or cell lines that uses isobaric tags (TMT (tandem mass tags)-10) for multiplexed …
Glucocorticoids promote breast cancer metastasis
Diversity within or between tumours and metastases (known as intra-patient tumour
heterogeneity) that develops during disease progression is a serious hurdle for therapy …
heterogeneity) that develops during disease progression is a serious hurdle for therapy …
Restoration of energy homeostasis by SIRT6 extends healthy lifespan
Aging leads to a gradual decline in physical activity and disrupted energy homeostasis. The
NAD+-dependent SIRT6 deacylase regulates aging and metabolism through mechanisms …
NAD+-dependent SIRT6 deacylase regulates aging and metabolism through mechanisms …
Organoid models of human and mouse ductal pancreatic cancer
Pancreatic cancer is one of the most lethal malignancies due to its late diagnosis and limited
response to treatment. Tractable methods to identify and interrogate pathways involved in …
response to treatment. Tractable methods to identify and interrogate pathways involved in …
A draft map of the human proteome
The availability of human genome sequence has transformed biomedical research over the
past decade. However, an equivalent map for the human proteome with direct …
past decade. However, an equivalent map for the human proteome with direct …
High-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform
Mass spectrometry has transformed the field of cell signaling by enabling global studies of
dynamic protein phosphorylation ('phosphoproteomics'). Recent developments are enabling …
dynamic protein phosphorylation ('phosphoproteomics'). Recent developments are enabling …
DCAF1-based PROTACs with activity against clinically validated targets overcoming intrinsic-and acquired-degrader resistance
Targeted protein degradation (TPD) mediates protein level through small molecule induced
redirection of E3 ligases to ubiquitinate neo-substrates and mark them for proteasomal …
redirection of E3 ligases to ubiquitinate neo-substrates and mark them for proteasomal …