Sequencing technologies and genome sequencing
The high-throughput-next generation sequencing (HT-NGS) technologies are currently the
hottest topic in the field of human and animals genomics researches, which can produce …
hottest topic in the field of human and animals genomics researches, which can produce …
A window into third-generation sequencing
First-and second-generation sequencing technologies have led the way in revolutionizing
the field of genomics and beyond, motivating an astonishing number of scientific advances …
the field of genomics and beyond, motivating an astonishing number of scientific advances …
Sequencing error profiles of Illumina sequencing instruments
Sequencing technology has achieved great advances in the past decade. Studies have
previously shown the quality of specific instruments in controlled conditions. Here, we …
previously shown the quality of specific instruments in controlled conditions. Here, we …
TAIL-seq: genome-wide determination of poly (A) tail length and 3′ end modifications
Global investigation of the 3′ extremity of mRNA (3′-terminome), despite its importance in
gene regulation, has not been feasible due to technical challenges associated with …
gene regulation, has not been feasible due to technical challenges associated with …
RNA-seq: from technology to biology
Next-generation sequencing technologies are now being exploited not only to analyse static
genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these …
genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these …
Sequencing by avidity enables high accuracy with low reagent consumption
S Arslan, FJ Garcia, M Guo, MW Kellinger… - Nature …, 2024 - nature.com
We present avidity sequencing, a sequencing chemistry that separately optimizes the
processes of step** along a DNA template and that of identifying each nucleotide within …
processes of step** along a DNA template and that of identifying each nucleotide within …
The role of replicates for error mitigation in next-generation sequencing
Advances in next-generation sequencing (NGS) technologies have rapidly improved
sequencing fidelity and substantially decreased sequencing error rates. However, given that …
sequencing fidelity and substantially decreased sequencing error rates. However, given that …
Reviving the transcriptome studies: an insight into the emergence of single-molecule transcriptome sequencing
Advances in transcriptomics have provided an exceptional opportunity to study functional
implications of the genetic variability. Technologies such as RNA-Seq have emerged as …
implications of the genetic variability. Technologies such as RNA-Seq have emerged as …
Bias in RNA‐seq library preparation: current challenges and solutions
H Shi, Y Zhou, E Jia, M Pan, Y Bai… - BioMed research …, 2021 - Wiley Online Library
Although RNA sequencing (RNA‐seq) has become the most advanced technology for
transcriptome analysis, it also confronts various challenges. As we all know, the workflow of …
transcriptome analysis, it also confronts various challenges. As we all know, the workflow of …
Microbiome profiling by illumina sequencing of combinatorial sequence-tagged PCR products
We developed a low-cost, high-throughput microbiome profiling method that uses
combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region …
combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region …