Sequencing technologies and genome sequencing

CS Pareek, R Smoczynski, A Tretyn - Journal of applied genetics, 2011 - Springer
The high-throughput-next generation sequencing (HT-NGS) technologies are currently the
hottest topic in the field of human and animals genomics researches, which can produce …

A window into third-generation sequencing

EE Schadt, S Turner, A Kasarskis - Human molecular genetics, 2010 - academic.oup.com
First-and second-generation sequencing technologies have led the way in revolutionizing
the field of genomics and beyond, motivating an astonishing number of scientific advances …

Sequencing error profiles of Illumina sequencing instruments

N Stoler, A Nekrutenko - NAR genomics and bioinformatics, 2021 - academic.oup.com
Sequencing technology has achieved great advances in the past decade. Studies have
previously shown the quality of specific instruments in controlled conditions. Here, we …

TAIL-seq: genome-wide determination of poly (A) tail length and 3′ end modifications

H Chang, J Lim, M Ha, VN Kim - Molecular cell, 2014 - cell.com
Global investigation of the 3′ extremity of mRNA (3′-terminome), despite its importance in
gene regulation, has not been feasible due to technical challenges associated with …

RNA-seq: from technology to biology

S Marguerat, J Bähler - Cellular and molecular life sciences, 2010 - Springer
Next-generation sequencing technologies are now being exploited not only to analyse static
genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these …

Sequencing by avidity enables high accuracy with low reagent consumption

S Arslan, FJ Garcia, M Guo, MW Kellinger… - Nature …, 2024 - nature.com
We present avidity sequencing, a sequencing chemistry that separately optimizes the
processes of step** along a DNA template and that of identifying each nucleotide within …

The role of replicates for error mitigation in next-generation sequencing

K Robasky, NE Lewis, GM Church - Nature Reviews Genetics, 2014 - nature.com
Advances in next-generation sequencing (NGS) technologies have rapidly improved
sequencing fidelity and substantially decreased sequencing error rates. However, given that …

Reviving the transcriptome studies: an insight into the emergence of single-molecule transcriptome sequencing

B Wang, V Kumar, A Olson, D Ware - Frontiers in genetics, 2019 - frontiersin.org
Advances in transcriptomics have provided an exceptional opportunity to study functional
implications of the genetic variability. Technologies such as RNA-Seq have emerged as …

Bias in RNA‐seq library preparation: current challenges and solutions

H Shi, Y Zhou, E Jia, M Pan, Y Bai… - BioMed research …, 2021 - Wiley Online Library
Although RNA sequencing (RNA‐seq) has become the most advanced technology for
transcriptome analysis, it also confronts various challenges. As we all know, the workflow of …

Microbiome profiling by illumina sequencing of combinatorial sequence-tagged PCR products

GB Gloor, R Hummelen, JM Macklaim, RJ Dickson… - PloS one, 2010 - journals.plos.org
We developed a low-cost, high-throughput microbiome profiling method that uses
combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region …