Single molecule real-time (SMRT) sequencing comes of age: applications and utilities for medical diagnostics
Short read massive parallel sequencing has emerged as a standard diagnostic tool in the
medical setting. However, short read technologies have inherent limitations such as GC …
medical setting. However, short read technologies have inherent limitations such as GC …
From the pipeline to the bedside: advances and challenges in clinical metagenomics
A Dulanto Chiang, JP Dekker - The Journal of infectious …, 2020 - academic.oup.com
Next-generation sequencing (NGS) technologies have revolutionized multiple areas in the
field of infectious diseases, from pathogen discovery to characterization of genes mediating …
field of infectious diseases, from pathogen discovery to characterization of genes mediating …
Long-read sequencing–a powerful tool in viral transcriptome research
Long-read sequencing (LRS) has become increasingly popular due to its strengths in de
novo assembly and in resolving complex DNA regions as well as in determining full-length …
novo assembly and in resolving complex DNA regions as well as in determining full-length …
[HTML][HTML] Recent advances in inferring viral diversity from high-throughput sequencing data
Rapidly evolving RNA viruses prevail within a host as a collection of closely related variants,
referred to as viral quasispecies. Advances in high-throughput sequencing (HTS) …
referred to as viral quasispecies. Advances in high-throughput sequencing (HTS) …
HIV evolution and diversity in ART-treated patients
Characterizing HIV genetic diversity and evolution during antiretroviral therapy (ART)
provides insights into the mechanisms that maintain the viral reservoir during ART. This …
provides insights into the mechanisms that maintain the viral reservoir during ART. This …
[HTML][HTML] HIV-1 variants are archived throughout infection and persist in the reservoir
The HIV-1 reservoir consists of latently infected cells that persist despite antiretroviral
therapy (ART). Elucidating the proviral genetic composition of the reservoir, particularly in …
therapy (ART). Elucidating the proviral genetic composition of the reservoir, particularly in …
Heterosexual transmission of subtype C HIV-1 selects consensus-like variants without increased replicative capacity or interferon-α resistance
Heterosexual transmission of HIV-1 is characterized by a genetic bottleneck that selects a
single viral variant, the transmitted/founder (TF), during most transmission events. To assess …
single viral variant, the transmitted/founder (TF), during most transmission events. To assess …
Future technologies for monitoring HIV drug resistance and cure
Current population genoty** methods for resistance monitoring are high cost and low
throughput. NGS, combined with simpler sample collection and storage matrices (eg dried …
throughput. NGS, combined with simpler sample collection and storage matrices (eg dried …
Analysis of transcripts and splice isoforms in Medicago sativa L. by single-molecule long-read sequencing
Y Chao, J Yuan, T Guo, L Xu, Z Mu, L Han - Plant Molecular Biology, 2019 - Springer
Key message The full-length transcriptome of alfalfa was analyzed with PacBio single-
molecule long-read sequencing technology. The transcriptome data provided full-length …
molecule long-read sequencing technology. The transcriptome data provided full-length …
Assessment of the current status of potyviruses in watermelon and pumpkin crops in Spain: Epidemiological impact of cultivated plants and mixed infections
Viral infections on cucurbit plants cause substantial quality and yield losses on their crops.
The diseased plants can often be infected by multiple viruses, and their epidemiology may …
The diseased plants can often be infected by multiple viruses, and their epidemiology may …