Bacterial whole-cell biocatalysts by surface display of enzymes: toward industrial application

J Schüürmann, P Quehl, G Festel, J Jose - Applied microbiology and …, 2014‏ - Springer
Despite the first report on the bacterial display of a recombinant peptide appeared almost 30
years ago, industrial application of cells with surface-displayed enzymes is still limited. To …

Bacterial serine proteases secreted by the autotransporter pathway: classification, specificity, and role in virulence

F Ruiz-Perez, JP Nataro - Cellular and molecular life sciences, 2014‏ - Springer
Serine proteases exist in eukaryotic and prokaryotic organisms and have emerged during
evolution as the most abundant and functionally diverse group. In Gram-negative bacteria …

The antigen 43 structure reveals a molecular Velcro-like mechanism of autotransporter-mediated bacterial clum**

B Heras, M Totsika, KM Peters, JJ Paxman… - Proceedings of the …, 2014‏ - pnas.org
Aggregation and biofilm formation are critical mechanisms for bacterial resistance to host
immune factors and antibiotics. Autotransporter (AT) proteins, which represent the largest …

Type V secretion systems in bacteria

E Fan, N Chauhan, DG Udatha… - … of Bacterial Pathogens, 2016‏ - Wiley Online Library
Gram‐negative bacteria are surrounded by two membranes, called the outer and inner
membranes. The space between these membranes, the periplasm, is spanned by a …

[HTML][HTML] Type V secretion: from biogenesis to biotechnology

P Van Ulsen, S ur Rahman, WSP Jong… - … et Biophysica Acta (BBA …, 2014‏ - Elsevier
The two membranes of Gram-negative bacteria contain protein machines that have a
general function in their assembly. To interact with the extra-cellular milieu, Gram-negatives …

Phylogenetic classification and functional review of autotransporters

KR Clarke, L Hor, A Pilapitiya, J Luirink… - Frontiers in …, 2022‏ - frontiersin.org
Autotransporters are the core component of a molecular nano-machine that delivers cargo
proteins across the outer membrane of Gram-negative bacteria. Part of the type V secretion …

Properties of protein unfolded states suggest broad selection for expanded conformational ensembles

MA Bowman, JA Riback, A Rodriguez, H Guo… - Proceedings of the …, 2020‏ - pnas.org
Much attention is being paid to conformational biases in the ensembles of intrinsically
disordered proteins. However, it is currently unknown whether or how conformational biases …

The ABCs and 123s of bacterial secretion systems in plant pathogenesis

JH Chang, D Desveaux… - Annual review of …, 2014‏ - annualreviews.org
Bacteria have many export and secretion systems that translocate cargo into and across
biological membranes. Seven secretion systems contribute to pathogenicity by translocating …

BamA forms a translocation channel for polypeptide export across the bacterial outer membrane

MT Doyle, HD Bernstein - Molecular cell, 2021‏ - cell.com
The β-barrel assembly machine (BAM) integrates β-barrel proteins into the outer membrane
(OM) of Gram-negative bacteria. An essential BAM subunit (BamA) catalyzes integration by …

Autotransporter-based cell surface display in Gram-negative bacteria

T Nicolay, J Vanderleyden… - Critical reviews in …, 2015‏ - Taylor & Francis
Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …