Nanosecond pulsed electric field (nsPEF): Opening the biotechnological Pandora's box

AR Ruiz-Fernández, L Campos… - International Journal of …, 2022 - mdpi.com
Nanosecond Pulsed Electric Field (nsPEF) is an electrostimulation technique first developed
in 1995; nsPEF requires the delivery of a series of pulses of high electric fields in the order of …

Electroporation and cell killing by milli-to nanosecond pulses and avoiding neuromuscular stimulation in cancer ablation

E Gudvangen, V Kim, V Novickij, F Battista… - Scientific Reports, 2022 - nature.com
Ablation therapies aim at eradication of tumors with minimal impact on surrounding healthy
tissues. Conventional pulsed electric field (PEF) treatments cause pain and muscle …

Perspective on pulsed electric field treatment in the bio-based industry

L Buchmann, A Mathys - Frontiers in bioengineering and …, 2019 - frontiersin.org
The bio-based industry is urged to find solutions to meet the demands of a growing world
population. In this context, increased resource efficiency is a major goal. Pulsed electric field …

Nanosecond pulsed electric fields induce endoplasmic reticulum stress accompanied by immunogenic cell death in murine models of lymphoma and colorectal …

A Rossi, O N. Pakhomova, PA Mollica, M Casciola… - Cancers, 2019 - mdpi.com
Depending on the initiating stimulus, cancer cell death can be immunogenic or non-
immunogenic. Inducers of immunogenic cell death (ICD) rely on endoplasmic reticulum (ER) …

Human cardiomyocytes are more susceptible to irreversible electroporation by pulsed electric field than human esophageal cells

M Casciola, D Keck, TK Feaster… - Physiological …, 2022 - Wiley Online Library
Pulse electric field‐based (PEF) ablation is a technique whereby short high‐intensity electric
fields inducing irreversible electroporation (IRE) are applied to various tissues. Here, we …

Susceptibility of various human cancer cell lines to nanosecond and microsecond range electrochemotherapy: Feasibility of multi-drug cocktails

N Rembiałkowska, V Novickij… - International Journal of …, 2023 - Elsevier
Electrochemotherapy (ECT) involves combining anticancer drugs with electroporation,
which is induced by pulsed electric fields (PEFs), while the effects vary in effectiveness …

Pulse width and intensity effects of pulsed electric fields on cancerous and normal skin cells

X Rao, S Chen, Y Alfadhl, X Chen, L Sun, L Yu… - Scientific Reports, 2022 - nature.com
Microsecond pulsed electric fields (PEF) have previously been used for various tumour
therapies, such as gene therapy, electrochemotherapy and irreversible electroporation …

Excitation and electroporation by MHz bursts of nanosecond stimuli

AG Pakhomov, S **ao, V Novickij, M Casciola… - Biochemical and …, 2019 - Elsevier
Intense nanosecond pulsed electric field (nsPEF) is a novel modality for cell activation and
nanoelectroporation. Applications of nsPEF in research and therapy are hindered by a high …

[HTML][HTML] Expression of voltage-gated calcium channels augments cell susceptibility to membrane disruption by nanosecond pulsed electric field

K Hristov, U Mangalanathan, M Casciola… - … et Biophysica Acta (BBA …, 2018 - Elsevier
We compared membrane permeabilization by nanosecond pulsed electric field (nsPEF) in
HEK293 cells with and without assembled CaV1. 3 L-type voltage-gated calcium channel …

Calcium electroporation causes ATP depletion in cells and is effective both in microsecond and nanosecond pulse range as a modality of electrochemotherapy

Calcium electroporation is a modality of electrochemotherapy (ECT), which is based on
intracellular electric field-mediated delivery of cytotoxic doses of calcium into the cells …