Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …

Advancements in Genome editing technologies have revolutionized the fields of functional
genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short …

We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …

The combined availability of whole genome sequences and genome editing tools is set to
revolutionize the field of fruit biotechnology by enabling the introduction of targeted genetic …

Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of
plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV …

Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome
editing system are usually mosaics in the T1 generation. In this study, we used egg cell …

Targeted genome editing using artificial nucleases has the potential to accelerate basic
research as well as plant breeding by providing the means to modify genomes rapidly in a …

Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …

Fruits are major sources of essential nutrients and serve as staple foods in some areas of
the world. The increasing human population and changes in climate experienced worldwide …

The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …