CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

The vast majority of missense variants observed in the human genome are of unknown
clinical significance. We present AlphaMissense, an adaptation of AlphaFold fine-tuned on …

Advances in molecular profiling have opened up the possibility to map the expression of
genes in cells, tissues, and organs in the human body. Here, we combined single-cell …

SARS-CoV-2 is associated with broad tissue tropism, a characteristic often determined by
the availability of entry receptors on host cells. Here, we show that TMEM106B, a lysosomal …

The human proteome contains approximately 20,000 proteins, and it is estimated that more
than 600 of them are functionally important for various types of cancers, including nearly 400 …

Tumour cells have exquisite flexibility in reprogramming their metabolism in order to support
tumour initiation, progression, metastasis and resistance to therapies. These reprogrammed …

Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …

Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …

Identification of host genes essential for severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) infection may reveal novel therapeutic targets and inform our understanding …