Subcellular organization: a critical feature of bacterial cell replication

IV Surovtsev, C Jacobs-Wagner - Cell, 2018‏ - cell.com
Spatial organization is a hallmark of all living systems. Even bacteria, the smallest forms of
cellular life, display defined shapes and complex internal organization, showcasing a highly …

Coupled transcription-translation in prokaryotes: an old couple with new surprises

M Irastortza-Olaziregi, O Amster-Choder - Frontiers in Microbiology, 2021‏ - frontiersin.org
Coupled transcription-translation (CTT) is a hallmark of prokaryotic gene expression. CTT
occurs when ribosomes associate with and initiate translation of mRNAs whose transcription …

Molecular dynamics simulation of an entire cell

JA Stevens, F Grünewald, PAM van Tilburg… - Frontiers in …, 2023‏ - frontiersin.org
The ultimate microscope, directed at a cell, would reveal the dynamics of all the cell's
components with atomic resolution. In contrast to their real-world counterparts …

Superresolution imaging of ribosomes and RNA polymerase in live Escherichia coli cells

S Bakshi, A Siryaporn, M Goulian… - Molecular …, 2012‏ - Wiley Online Library
Quantitative spatial distributions of ribosomes (S2‐YFP) and RNA polymerase (RNAP; β′‐
yGFP) in live Escherichia coli are measured by superresolution fluorescence microscopy. In …

Live-cell superresolution microscopy reveals the organization of RNA polymerase in the bacterial nucleoid

M Stracy, C Lesterlin, F Garza de Leon… - Proceedings of the …, 2015‏ - pnas.org
Despite the fundamental importance of transcription, a comprehensive analysis of RNA
polymerase (RNAP) behavior and its role in the nucleoid organization in vivo is lacking …

Single-particle tracking reveals that free ribosomal subunits are not excluded from the Escherichia coli nucleoid

A Sanamrad, F Persson, EG Lundius, D Fange… - Proceedings of the …, 2014‏ - pnas.org
Biochemical and genetic data show that ribosomes closely follow RNA polymerases that are
transcribing protein-coding genes in bacteria. At the same time, electron and fluorescence …

Interconnecting solvent quality, transcription, and chromosome folding in Escherichia coli

Y **ang, IV Surovtsev, Y Chang, SK Govers, BR Parry… - Cell, 2021‏ - cell.com
All cells fold their genomes, including bacterial cells, where the chromosome is compacted
into a domain-organized meshwork called the nucleoid. How compaction and domain …

The spatial biology of transcription and translation in rapidly growing Escherichia coli

S Bakshi, H Choi, JC Weisshaar - Frontiers in microbiology, 2015‏ - frontiersin.org
Single-molecule fluorescence provides high resolution spatial distributions of ribosomes
and RNA polymerase (RNAP) in live, rapidly growing Escherichia coli. Ribosomes are more …

Membrane-localized expression, production and assembly of Vibrio parahaemolyticus T3SS2 provides evidence for transertion

KG Kaval, S Chimalapati, SD Siegel, N Garcia… - Nature …, 2023‏ - nature.com
It has been proposed that bacterial membrane proteins may be synthesized and inserted
into the membrane by a process known as transertion, which involves membrane …

Localization of Protein Aggregation in Escherichia coli Is Governed by Diffusion and Nucleoid Macromolecular Crowding Effect

AS Coquel, JP Jacob, M Primet… - PLoS computational …, 2013‏ - journals.plos.org
Aggregates of misfolded proteins are a hallmark of many age-related diseases. Recently,
they have been linked to aging of Escherichia coli (E. coli) where protein aggregates …