Green synthesis aspects of (R)-(-)-mandelic acid; a potent pharmaceutically active agent and its future prospects
RV Singh, K Sambyal - Critical Reviews in Biotechnology, 2023 - Taylor & Francis
Abstract (R)-(-)-mandelic acid is an important carboxylic acid known for its numerous
potential applications in the pharmaceutical industry as it is an ideal starting material for the …
potential applications in the pharmaceutical industry as it is an ideal starting material for the …
Microbial transformation of nitriles to high-value acids or amides
J Chen, RC Zheng, YG Zheng, YC Shen - … in China I: From Bioreaction to …, 2009 - Springer
Biotransformation of nitriles mediated by nitrile-amide converting enzymes has attracted
considerable attention and developed tremendously in the recent years in China since it …
considerable attention and developed tremendously in the recent years in China since it …
Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401
YC He, JH Xu, JH Su, L Zhou - Applied Biochemistry and Biotechnology, 2010 - Springer
Alcaligenes sp. ECU0401 has been isolated from soil samples with high nitrilase activity
against glycolonitrile using the enrichment culture technique. The preferred carbon/nitrogen …
against glycolonitrile using the enrichment culture technique. The preferred carbon/nitrogen …
The combi-CLEA approach: enzymatic cascade synthesis of enantiomerically pure (S)-mandelic acid
A Chmura, S Rustler, M Paravidino, F van Rantwijk… - Tetrahedron …, 2013 - Elsevier
Enantiomerically pure (S)-mandelic acid was synthesised from benzaldehyde by sequential
hydrocyanation and hydrolysis in a bienzymatic cascade at starting concentrations up to …
hydrocyanation and hydrolysis in a bienzymatic cascade at starting concentrations up to …
Improvement of Alcaligenes faecalis Nitrilase by Gene Site Saturation Mutagenesis and Its Application in Stereospecific Biosynthesis of (R)-(−)-Mandelic Acid
Nitrilases have recently received considerable attention as the biocatalysts for stereospecific
production of carboxylic acids. To improve the activity, the nitrilase from Alcaligenes faecalis …
production of carboxylic acids. To improve the activity, the nitrilase from Alcaligenes faecalis …
Whole Cell‐Based Cascade Biotransformation for the Production of (S)‐Mandelic Acid from Styrene, L‐Phenylalanine, Glucose, or Glycerol
Abstract (S)‐Mandelic acid is a useful and high value chemical with many synthetic
applications, but its synthesis often requires the use of toxic cyanide. Here, we report the …
applications, but its synthesis often requires the use of toxic cyanide. Here, we report the …
Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(−)-mandelic acid …
ZJ Zhang, JH Xu, YC He, LM Ouyang… - Bioprocess and biosystems …, 2011 - Springer
A nitrilase gene from Alcaligenes sp. ECU0401 was cloned and overexpressed in
Escherichia coli BL21 (DE3) in a soluble form. The encoded protein with a His 6-tag was …
Escherichia coli BL21 (DE3) in a soluble form. The encoded protein with a His 6-tag was …
Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene–water biphasic system
ZJ Zhang, J Pan, JF Liu, JH Xu, YC He, YY Liu - Journal of biotechnology, 2011 - Elsevier
The enantioselective hydrolysis of mandelonitrile with whole cells of a recombinant
Escherichia coli expressing nitrilase activity was severely inhibited by the substrate at high …
Escherichia coli expressing nitrilase activity was severely inhibited by the substrate at high …
Efficient production of (R)-(−)-mandelic acid with highly substrate/product tolerant and enantioselective nitrilase of recombinant Alcaligenes sp.
ZJ Zhang, JH Xu, YC He, LM Ouyang, YY Liu… - Process …, 2010 - Elsevier
For efficient production of (R)-(−)-mandelic acid, a nitrilase gene from Alcaligenes sp.
ECU0401 was cloned and overexpressed in Escherichia coli. After simple optimization of …
ECU0401 was cloned and overexpressed in Escherichia coli. After simple optimization of …
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications
SP Zhou, YP Xue, YG Zheng - Biotechnology Advances, 2024 - Elsevier
Nitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the
hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids …
hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids …