CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane

GS Prado, DC Rocha, LN Santos… - Frontiers in Plant …, 2024 - frontiersin.org
Gene editing technologies have opened up the possibility of manipulating the genome of
any organism in a predicted way. CRISPR technology is the most used genome editing tool …

CRISPR-Cas engineering in food science and sustainable agriculture: recent advancements and applications

M Aman Mohammadi, MR Maximiano… - Bioprocess and …, 2023 - Springer
The developments in the food supply chain to support the growing population of the world is
one of today's most pressing issues, and to achieve this goal improvements should be …

CRISPR/Cas9-Mediated Targeted Mutagenesis of FtMYB45 Promotes Flavonoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum)

D Wen, L Wu, M Wang, W Yang, X Wang… - Frontiers in plant …, 2022 - frontiersin.org
The clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9
(CRISPR/Cas9) technology is an efficient genome editing tool used in multiple plant …

A transcriptional cascade involving BBX22 and HY5 finely regulates both plant height and fruit pigmentation in citrus

J Fu, L Liao, J **, Z Lu, J Sun, L Song… - Journal of Integrative …, 2024 - Wiley Online Library
Dwarfing is a pivotal agronomic trait affecting both yield and quality. Citrus species exhibit
substantial variation in plant height, among which internode length is a core element …

An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus

CHD Sagawa, G Thomson, B Mermaz, C Vernon, S Liu… - Plant Methods, 2024 - Springer
CRISPR/Cas9-mediated gene editing requires high efficiency to be routinely implemented,
especially in species which are laborious and slow to transform. This requirement intensifies …

CRISPR/Cas9 editing characteristics of multiple transgenic generations in Fortunella hindsii, an early flowering mini-citrus

X Song, Y **e, X Tian, N Wang, Y Zhou, Z **e, J Ye… - Scientia …, 2023 - Elsevier
The CRISPR/Cas9 editing system is presently regarded as one of the most efficient tools for
genome editing and has been implemented in perennial fruit crops. However, the …

[HTML][HTML] Heat stress increases mutation efficiency mediated by CRISPR/Cas9 in citrus

A Peng, Z Chen, Y Zhu, Z Ye, X Zou, Y He, Q Li… - Horticultural Plant …, 2024 - Elsevier
The CRISPR/Cas9 system has shown great promise in engineering targeted mutations in a
genome. The efficiency of Cas9-mediated genome editing is temperature sensitive. A high …

Development and application of gene editing in citrus: how and what to do

S Wang, S Zhao, M Du, K Mo, S Pang, X Zou - Horticulture Advances, 2024 - Springer
Conventional breeding techniques have been effectively utilized for the enhancement of
citrus varieties. Nonetheless, traits such as an extended juvenile phase, cross-or self …

[PDF][PDF] Huanglongbing (HLB) and its vectors: recent research advances and future challenges

M Pérez-Hedo, MS Hoddle, F Alferez, A Tena, T Wade… - 2024 - researchgate.net
Huanglongbing (HLB), also known as citrus greening disease, is one of the most destructive
diseases affecting the global citrus industry. It is caused by bacteria of the Candidatus …

Efficient targeted mutagenesis by endogenous promoter controlled tRNA-gRNA array of CRISPR/Cas9 in potato and application in Solanum etuberosum

S **g, W **ong, H Liu, F Dong, K Chen, J Du, B Song - 2022 - researchsquare.com
Here, we developed an endogenous promoter controlled polycistronic tRNA-gRNA (PTG)
array of CRISPR/Cas9 in potato for efficient multiplex mutagenesis. The editing efficiency …