Bacteria and their viruses have coevolved for billions of years. This ancient and still ongoing
arms race has led bacteria to develop a vast antiphage arsenal. The development of high …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Retrons are prokaryotic genetic retroelements encoding a reverse transcriptase that
produces multi-copy single-stranded DNA (msDNA). Despite decades of research on the …

Continuous evolution can generate biomolecules for synthetic biology and enable
evolutionary investigation. The orthogonal DNA replication system (OrthoRep) in yeast can …

Whole-genome synthesis provides a powerful approach for understanding and expanding
organism function,–. To build large genomes rapidly, scalably and in parallel, we need (1) …

CRISPR base editing is a powerful method to engineer bacterial genomes. However, it
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …

Bacteriophages are the most abundant biological entity in the world and hold a tremendous
amount of unexplored genetic information. Since their discovery, phages have drawn a great …

Exogenous DNA can be a template to precisely edit a cell's genome. However, the delivery
of in vitro-produced DNA to target cells can be inefficient, and low abundance of template …

Recombination-mediated genetic engineering, also known as recombineering, is the
genomic incorporation of homologous single-stranded or double-stranded DNA into …

The safety issues like immunogenicity and unacceptable cancer risk of viral vectors for
DNA/mRNA vaccine delivery necessitate the development of non-viral vectors with no …