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STI PCR: An efficient method for amplification and de novo synthesis of long DNA sequences
Z Zhao, X **e, W Liu, J Huang, J Tan, H Yu, W Zong… - Molecular Plant, 2022 - cell.com
Despite continuous improvements, it is difficult to efficiently amplify large sequences from
complex templates using current PCR methods. Here, we developed a suppression thermo …
complex templates using current PCR methods. Here, we developed a suppression thermo …
DNA extraction from plant leaves using a microneedle patch
Isolation of high‐quality DNA from infected plant specimens is an essential step for the
molecular detection of plant pathogens. However, DNA isolation from plant cells surrounded …
molecular detection of plant pathogens. However, DNA isolation from plant cells surrounded …
[HTML][HTML] Isolating Linum usitatissimum L. Nuclear DNA Enabled Assembling High-Quality Genome
High-quality genome sequences help to elucidate the genetic basis of numerous biological
processes and track species evolution. For flax (Linum usitatissimum L.)—a multifunctional …
processes and track species evolution. For flax (Linum usitatissimum L.)—a multifunctional …
The phylogenetic analysis of Dalbergia (Fabaceae: Papilionaceae) based on different DNA barcodes
The genus Dalbergia contains approximately 250 species with many valuable trees being
destroyed by targeted and illegal logging. DNA barcoding is a reliable method for the …
destroyed by targeted and illegal logging. DNA barcoding is a reliable method for the …
FLASH Genome Editing Pipeline: An Efficient and High‐Throughput Method to Construct Arrayed CRISPR Library for Plant Functional Genomics
L Yao, X Wang, R Ke, K Chen, K **e - Current Protocols, 2023 - Wiley Online Library
CRISPR/Cas9 genome editing is a revolutionary technology for plant functional genomics
and crop breeding. In this system, the Cas9 nuclease is directed by a guide RNA (gRNA) to …
and crop breeding. In this system, the Cas9 nuclease is directed by a guide RNA (gRNA) to …
Absolute quantitation of plant proteins
Among targeted proteomic techniques, AQUA‐MRM is considered as one of the most
reliable for accurate protein quantitation. This method displays high sensitivity, specificity …
reliable for accurate protein quantitation. This method displays high sensitivity, specificity …