Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

Cell-free protein synthesis (CFPS) is an in vitro process that enables diverse applications in
research, biomanufacturing, point-of-care diagnostics, therapeutics, and education using …

Cell‐free protein synthesis (CFPS) is an established method for rapid recombinant protein
production. Advantages like short synthesis times and an open reaction environment make …

Recent studies have shown that the reconstituted cell-free DNA replisome and in vitro
transcription and translation systems from Escherichia coli are highly important in applied …

DNA templates for protein production remain an unexplored source of variability in the
performance of cell-free expression (CFE) systems. To characterize this variability, we …

The optimization and control of cell free protein synthesis (CFPS) presents an ongoing
challenge due to the complex synergies and nonlinearities that cannot be fully explained in …

Abstract Cell‐Free Protein Synthesis (CFPS) has, over the past decade, seen a substantial
increase in interest from both academia and industry. Applications range from fundamental …

Performance variability is a common challenge in cell-free protein production and hinders a
wider adoption of these systems for both research and biomanufacturing. While the inherent …

The capacity to utilize biochemical machinery to produce proteins in vitro has become an
increasingly accessible and easy to use platform for molecular biology applications. Over …

The work described in this report was authorized under the fiscal year 2019 Innovative
Development of Employee Advanced Solutions Program entitled “Exploiting Designed …