Review of the recombinant human interferon gamma as an immunotherapeutic: Impacts of production platforms and glycosylation

A Razaghi, L Owens, K Heimann - Journal of biotechnology, 2016‏ - Elsevier
Human interferon gamma is a cytokine belonging to a diverse group of interferons which
have a crucial immunological function against mycobacteria and a wide variety of viral …

Trace metals in cellular metabolism and their impact on recombinant protein production

A Prabhu, M Gadgil - Process Biochemistry, 2021‏ - Elsevier
Replacement of serum and increasing use of chemically defined media demands
optimisation of trace metal components for biomanufacturing applications. Trace metal …

The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth …

M Jang, ES Pete, P Bruheim - Frontiers in Bioengineering and …, 2022‏ - frontiersin.org
Serum-free cultures are preferred for application in clinical cell therapy and facilitate the
purification processes of bioproducts, such as vaccines and recombinant proteins. It can …

A review: Analysis of technical challenges in cultured meat production and its commercialization

YY Zheng, ZN Hu, GH Zhou - Critical Reviews in Food Science and …, 2024‏ - Taylor & Francis
The cultured meat technology has developed rapidly in recent years, but there are still many
technical challenges that hinder the large-scale production and commercialization of …

Biosensors for waterborne viruses: Detection and removal

Z Altintas, M Gittens, J Pocock, IE Tothill - Biochimie, 2015‏ - Elsevier
Detection of waterborne viruses is important to eliminate and control their harmful effect as
pathogens. Hence, the use of rapid and sensitive detection technologies is critically …

Application of multi-omics techniques for bioprocess design and optimization in Chinese hamster ovary cells

A Farrell, N McLoughlin, JJ Milne… - Journal of proteome …, 2014‏ - ACS Publications
Significant improvements in the productivity and quality of therapeutic proteins produced in
Chinese hamster ovary (CHO) cells have been reported since their establishment as host …

Miniaturized single-cell technologies for monoclonal antibody discovery

J Van Lent, J Breukers, K Ven, L Ampofo, S Horta… - Lab on a Chip, 2021‏ - pubs.rsc.org
Antibodies (Abs) are among the most important class of biologicals, showcasing a high
therapeutic and diagnostic value. In the global therapeutic Ab market, fully-human …

[HTML][HTML] Transcriptomic changes in CHO cells after adaptation to suspension growth in protein-free medium analysed by a species-specific microarray

S Shridhar, G Klanert, N Auer, I Hernandez-Lopez… - Journal of …, 2017‏ - Elsevier
Abstract Chinese Hamster Ovary (CHO) cells are the preferred cell line for production of
biopharmaceuticals. These cells are capable to grow without serum supplementation, but …

The bioactivity and fractionation of peptide hydrolysates in cultures of CHO cells

M Spearman, C Lodewyks, M Richmond… - Biotechnology …, 2014‏ - Wiley Online Library
Peptide hydrolysate supplements in mammalian cell cultures provide enhanced growth and
productivity. The objective of this study was to compare the bioactivity of ten different …

Attenuation of glutamine synthetase selection marker improves product titer and reduces glutamine overflow in Chinese hamster ovary cells

SA Sacco, AM Tuckowski, I Trenary… - Biotechnology and …, 2022‏ - Wiley Online Library
The glutamine synthetase (GS) expression system is commonly used to ensure stable
transgene integration and amplification in Chinese hamster ovary (CHO) host lines …