Envisioning a new era: complete genetic information from routine, telomere-to-telomere genomes

KH Miga, EE Eichler - The American Journal of Human Genetics, 2023 - cell.com
Advances in long-read sequencing and assembly now mean that individual labs can
generate phased genomes that are more accurate and more contiguous than the original …

A signal processing and deep learning framework for methylation detection using Oxford Nanopore sequencing

MU Ahsan, A Gouru, J Chan, W Zhou… - Nature Communications, 2024 - nature.com
Oxford Nanopore sequencing can detect DNA methylations from ionic current signal of
single molecules, offering a unique advantage over conventional methods. Additionally …

DNA 5-methylcytosine detection and methylation phasing using PacBio circular consensus sequencing

P Ni, F Nie, Z Zhong, J Xu, N Huang, J Zhang… - Nature …, 2023 - nature.com
Long single-molecular sequencing technologies, such as PacBio circular consensus
sequencing (CCS) and nanopore sequencing, are advantageous in detecting DNA 5 …

Personalized recurrence risk assessment following the birth of a child with a pathogenic de novo mutation

M Bernkopf, UB Abdullah, SJ Bush, KA Wood… - Nature …, 2023 - nature.com
Following the diagnosis of a paediatric disorder caused by an apparently de novo mutation,
a recurrence risk of 1–2% is frequently quoted due to the possibility of parental germline …

Shedding light on DNA methylation and its clinical implications: the impact of long-read-based nanopore technology

A Chera, M Stancu-Cretu, NR Zabet, O Bucur - Epigenetics & Chromatin, 2024 - Springer
DNA methylation is an essential epigenetic mechanism for regulation of gene expression,
through which many physiological (X-chromosome inactivation, genetic imprinting …

MethPhaser: methylation-based long-read haplotype phasing of human genomes

Y Fu, S Aganezov, M Mahmoud, J Beaulaurier… - Nature …, 2024 - nature.com
The assignment of variants across haplotypes, phasing, is crucial for predicting the
consequences, interaction, and inheritance of mutations and is a key step in improving our …

Graphasing: phasing diploid genome assembly graphs with single-cell strand sequencing

M Henglin, M Ghareghani, WT Harvey, D Porubsky… - Genome Biology, 2024 - Springer
Haplotype information is crucial for biomedical and population genetics research. However,
current strategies to produce de novo haplotype-resolved assemblies often require either …

Prognostic importance of direct assignment of parent-of-origin via long-read genome and epigenome sequencing in retinoblastoma

AW Stacey, K Nakamichi, J Huey, J Stevens… - JCI insight, 2024 - insight.jci.org
BACKGROUND. Current clinical sequencing methods cannot effectively detect DNA
methylation and allele-specific variation to provide parent-of-origin information from the …

High accuracy methylation identification tools on single molecular level for PacBio HiFi data

Y Chen, B Wu, Y Ding, L Niu, X Bai, Z Lin, CL **ao - bioRxiv, 2024 - biorxiv.org
PacBio Circular Consensus Sequencing (CCS) allows us to obtain highly accurate bases
and simultaneously determine the methylation states of individual molecules. However …

[HTML][HTML] Expanding Upon Genomics in Rare Diseases: Epigenomic Insights

JW Tan, EJ Blake, JD Farris, EW Klee - International journal of molecular …, 2024 - mdpi.com
DNA methylation is an essential epigenetic modification that plays a crucial role in
regulating gene expression and maintaining genomic stability. With the advancement in …