PCR technology for screening and quantification of genetically modified organisms (GMOs)

A Holst-Jensen, SB Rønning, A Løvseth… - Analytical and …, 2003 - Springer
Although PCR technology has obvious limitations, the potentially high degree of sensitivity
and specificity explains why it has been the first choice of most analytical laboratories …

Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products

E Anklam, F Gadani, P Heinze, H Pijnenburg… - … Food Research and …, 2002 - Springer
Numerous analytical methods, both qualitative and quantitative, have been developed to
determine reliably the presence and/or the amount of genetically modified organisms …

[BOOK][B] Handbook of food science, technology, and engineering

YH Hui - 2006 - books.google.com
Handbook of food science, technology, and engineering Page 1 HANDBOOK of FOOD
SCIENCE, TECHNOLOGY, AND ENGINEERING Volume 4 Edited by YH HUI Associate Editors …

Optimization of a high-throughput CTAB-based protocol for the extraction of qPCR-grade DNA from rumen fluid, plant and bacterial pure cultures

K Minas, NR McEwan, CJ Newbold… - FEMS microbiology …, 2011 - academic.oup.com
The quality and yield of extracted DNA are critical for the majority of downstream
applications in molecular biology. Moreover, molecular techniques such as quantitative real …

Effect of food processing on plant DNA degradation and PCR-based GMO analysis: a review

N Gryson - Analytical and bioanalytical chemistry, 2010 - Springer
The applicability of a DNA-based method for GMO detection and quantification depends on
the quality and quantity of the DNA. Important food-processing conditions, for example …

Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis

D James, A Schmidt, E Wall, M Green… - Journal of agricultural …, 2003 - ACS Publications
Multiplex PCR procedures were developed for simultaneously detecting multiple target
sequences in genetically modified (GM) soybean (Roundup Ready), maize (event 176, Bt11 …

Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods

M Vaitilingom, H Pijnenburg, F Gendre… - Journal of Agricultural …, 1999 - ACS Publications
A fast and quantitative method was developed to detect transgenic “Maximizer” maize “event
176”(Novartis) and “Roundup Ready” soybean (Monsanto) in food by real-time quantitative …

Testing for genetically modified organisms (GMOs): Past, present and future perspectives

A Holst-Jensen - Biotechnology advances, 2009 - Elsevier
This paper presents an overview of GMO testing methodologies and how these have
evolved and may evolve in the next decade. Challenges and limitations for the application of …

Validation of a Rice Specific Gene, Sucrose Phosphate Synthase, Used as the Endogenous Reference Gene for Qualitative and Real-Time Quantitative PCR …

J Ding, J Jia, L Yang, H Wen, C Zhang… - Journal of agricultural …, 2004 - ACS Publications
With the development of transgenic crops, many countries have issued regulations to label
the genetically modified organisms (GMOs) and their derived products. Polymerase Chain …

Pollen‐mediated gene flow in maize in real situations of coexistence

J Messeguer, G Peñas, J Ballester… - Plant Biotechnology …, 2006 - Wiley Online Library
We present the first study on cross‐fertilization between Bt and conventional maize in real
situations of coexistence in two regions in which Bt and conventional maize were cultivated …