The role of macrophages in cancer development and therapy

E Cendrowicz, Z Sas, E Bremer, TP Rygiel - Cancers, 2021 - mdpi.com
Simple Summary Tumor-Associated Macrophages (TAMs) play an important role in the
development of tumors, modulation of neoangiogenesis, immune suppression, and …

Off-target effects in CRISPR/Cas9-mediated genome engineering

XH Zhang, LY Tee, XG Wang, QS Huang… - … Therapy-Nucleic Acids, 2015 - cell.com
CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the
functionality of genetic elements, creating genetically modified organisms as well as …

Inference of CRISPR edits from Sanger trace data

D Conant, T Hsiau, N Rossi, J Oki, T Maures… - The CRISPR …, 2022 - liebertpub.com
Efficient and precise genome editing requires a fast, quantitative, and inexpensive assay to
assess genotype following editing. Here, we present ICE (Inference of CRISPR Edits), which …

Easy quantitative assessment of genome editing by sequence trace decomposition

EK Brinkman, T Chen, M Amendola… - Nucleic acids …, 2014 - academic.oup.com
The efficacy and the mutation spectrum of genome editing methods can vary substantially
depending on the targeted sequence. A simple, quick assay to accurately characterize and …

[HTML][HTML] The CRISPR/Cas9 system for plant genome editing and beyond

L Bortesi, R Fischer - Biotechnology advances, 2015 - Elsevier
Targeted genome editing using artificial nucleases has the potential to accelerate basic
research as well as plant breeding by providing the means to modify genomes rapidly in a …

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

D Kim, S Bae, J Park, E Kim, S Kim, HR Yu, J Hwang… - Nature …, 2015 - nature.com
Although RNA-guided genome editing via the CRISPR-Cas9 system is now widely used in
biomedical research, genome-wide target specificities of Cas9 nucleases remain …

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

S Bae, J Park, JS Kim - Bioinformatics, 2014 - academic.oup.com
The Type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system
is an adaptive immune response in prokaryotes, protecting host cells against invading …

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

S Kim, D Kim, SW Cho, J Kim, JS Kim - Genome research, 2014 - genome.cshlp.org
RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune
system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas …

CRISPR/Cas9 genome editing in wheat

D Kim, B Alptekin, H Budak - Functional & integrative genomics, 2018 - Springer
Genome editing has been a long-term challenge for molecular biology research, particularly
for plants possess complex genome. The recently discovered Clustered Regularly …

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

TG Montague, JM Cruz, JA Gagnon… - Nucleic acids …, 2014 - academic.oup.com
Major advances in genome editing have recently been made possible with the development
of the TALEN and CRISPR/Cas9 methods. The speed and ease of implementing these …