G protein-coupled receptors (GPCRs) are an extensive class of trans-plasma membrane
proteins that function to regulate a wide range of physiological functions. Despite a general …

G protein-coupled receptors (GPCRs) are organised within the cell membrane into highly
ordered macromolecular complexes along with other receptors and signalling proteins …

The exocyst is a conserved octameric complex that tethers exocytic vesicles to the plasma
membrane prior to fusion. Exocyst assembly and delivery mechanisms remain unclear …

Conspectus The cell plasma membrane (PM) contains thousands of proteins that sense and
respond to the outside environment. These proteins have evolved sensitivity to a wide …

G protein-coupled receptors (GPCRs) are a major gateway to cellular signaling, which
respond to ligands binding at extracellular sites through allosteric conformational changes …

Nonspecific interactions between lipids and fluorophores can alter the outcomes of single-
molecule spectroscopy of membrane proteins in live cells, liposomes or lipid nanodiscs and …

GPCRs have been shown to form oligomers, which generate distinctive signaling outcomes.
However, the structural nature of the oligomerization process remains uncertain. We have …

Understanding the photophysical properties of fluorescent proteins (FPs), such as emission
and absorption spectra, molecular brightness, photostability, and photo-switching, is critical …

Single-molecule counting techniques enable a precise determination of the intracellular
abundance and stoichiometry of proteins and macromolecular complexes. These details are …

G protein-coupled receptors (GPCRs) have been classically described as monomeric
entities that function by binding in a 1: 1 stoichiometric ratio to both ligand and downstream …