[HTML][HTML] The mRNA-LNP platform's lipid nanoparticle component used in preclinical vaccine studies is highly inflammatory

S Ndeupen, Z Qin, S Jacobsen, A Bouteau… - Iscience, 2021 - cell.com
Vaccines based on mRNA-containing lipid nanoparticles (LNPs) are a promising new
platform used by two leading vaccines against COVID-19. Clinical trials and ongoing …

A method for isolating and culturing skin cells: application to endothelial cells, fibroblasts, keratinocytes, and melanocytes from punch biopsies in systemic sclerosis …

P Henrot, P Laurent, E Levionnois, D Leleu… - Frontiers in …, 2020 - frontiersin.org
Systemic Sclerosis (SSc) is a complex auto-immune connective tissue disease combining
inflammatory, vasculopathic and fibrotic manifestations. Skin effectively recapitulates the …

Comparative analysis of antibody-and lipid-based multiplexing methods for single-cell RNA-seq

V Mylka, I Matetovici, S Poovathingal, J Aerts… - Genome Biology, 2022 - Springer
Background Multiplexing of samples in single-cell RNA-seq studies allows a significant
reduction of the experimental costs, straightforward identification of doublets, increased cell …

CD91 and its ligand gp96 confer cross-priming capabilities to multiple APCs during immune responses to nascent, emerging tumors

DA Nayak, AL Sedlacek, AR Cillo… - Cancer Immunology …, 2024 - aacrjournals.org
During cancer immunosurveillance, dendritic cells (DC) play a central role in orchestrating T-
cell responses against emerging tumors. Capture of miniscule amounts of antigen along …

IL‐23‐and imiquimod‐induced models of experimental psoriasis in mice

TP Singh, HH Zhang, ST Hwang… - Current protocols in …, 2019 - Wiley Online Library
Genome‐wide association studies have found that polymorphisms in genes for IL‐23 and its
receptor are important in psoriasis, and blocking IL‐23 is an effective therapy in the disease …

A spontaneous melanoma mouse model applicable for a longitudinal chemotherapy and immunotherapy study

K Eddy, K Gupta, JC Pelletier, AL Isola… - Journal of Investigative …, 2023 - Elsevier
Mouse models that reflect human disorders provide invaluable tools for the translation of
basic science discoveries to clinical therapies. However, many of these in vivo therapeutic …

[HTML][HTML] Bone marrow mesenchymal stem cells alleviate the formation of pathological scars in rats

JH Xu, SQ Xu, SL Ding, H Yang, X Huang, HF Shi - Regenerative Therapy, 2022 - Elsevier
Introduction Although bone marrow-derived mesenchymal stem cells (BMSCs) have
attracted increasing attention because of their pivotal functions in the process of wound …

Inhibition of inositol kinase B controls acute and chronic graft-versus-host disease

G Thangavelu, J Du, KG Paz, M Loschi… - Blood, The Journal …, 2020 - ashpublications.org
T-cell activation releases inositol 1, 4, 5-trisphosphate (IP3), inducing cytoplasmic calcium
(Ca2+) influx. In turn, inositol 1, 4, 5-trisphosphate 3-kinase B (Itpkb) phosphorylates IP3 to …

Altered ratio of dendritic cell subsets in skin-draining lymph nodes promotes Th2-driven contact hypersensitivity

HL Miller, PS Andhey, MK Swiecki… - Proceedings of the …, 2021 - National Acad Sciences
Plasmacytoid dendritic cells (pDCs) specialize in the production of type I IFN (IFN-I). pDCs
can be depleted in vivo by injecting diphtheria toxin (DT) in a mouse in which pDCs express …

[HTML][HTML] Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells

K Sakamoto, S Goel, A Funakoshi, T Honda, K Nagao - STAR protocols, 2022 - Elsevier
Skin is our body's outermost physical barrier and an immunological interface enriched with
various immune and non-immune cells. However, efficient generation of single-cell …