Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

INTRODUCTION The eukaryotic nucleus protects the genome and is enclosed by the two
membranes of the nuclear envelope. Nuclear pore complexes (NPCs) perforate the nuclear …

Mass spectrometry (MS) has become one of the key technologies of structural biology. In this
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …

INTRODUCTION The nucleus harbors and protects the genetic information in eukaryotes. It
is surrounded by the nuclear envelope, which separates the nucleoplasm from the cytosol …

Nuclear pore complexes (NPCs) create large conduits for cargo transport between the
nucleus and cytoplasm across the nuclear envelope (NE),–. These multi-megadalton …

Summary Human immunodeficiency virus (HIV-1) remains a major health threat. Viral capsid
uncoating and nuclear import of the viral genome are critical for productive infection. The …

Cryo-electron tomograms capture a wealth of structural information on the molecular
constituents of cells and tissues. We present DeePiCt (deep picker in context), an open …

Ribosomes catalyze protein synthesis by cycling through various functional states. These
states have been extensively characterized in vitro, but their distribution in actively …

INTRODUCTION Cytoplasmic face of the human NPC. Near-atomic composite structure of
the NPC generated by docking high-resolution crystal structures into a cryo‑ET …