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Mechanisms, applications, and challenges of insect RNA interference
The RNA interference (RNAi) triggered by short/small interfering RNA (siRNA) was
discovered in nematodes and found to function in most living organisms. RNAi has been …
discovered in nematodes and found to function in most living organisms. RNAi has been …
Argonaute proteins: functional insights and emerging roles
Small-RNA-guided gene regulation has emerged as one of the fundamental principles in
cell function, and the major protein players in this process are members of the Argonaute …
cell function, and the major protein players in this process are members of the Argonaute …
Genetics on the Fly: A Primer on the Drosophila Model System
Fruit flies of the genus Drosophila have been an attractive and effective genetic model
organism since Thomas Hunt Morgan and colleagues made seminal discoveries with them …
organism since Thomas Hunt Morgan and colleagues made seminal discoveries with them …
Diversity and dynamics of the Drosophila transcriptome
Animal transcriptomes are dynamic, with each cell type, tissue and organ system expressing
an ensemble of transcript isoforms that give rise to substantial diversity. Here we have …
an ensemble of transcript isoforms that give rise to substantial diversity. Here we have …
RISC assembly: Coordination between small RNAs and Argonaute proteins
Non-coding RNAs generally form ribonucleoprotein (RNP) complexes with their partner
proteins to exert their functions. Small RNAs, including microRNAs, small interfering RNAs …
proteins to exert their functions. Small RNAs, including microRNAs, small interfering RNAs …
Molecular mechanisms of Dicer: endonuclease and enzymatic activity
The enzyme Dicer is best known for its role as a riboendonuclease in the small RNA
pathway. In this canonical role, Dicer is a critical regulator of the biogenesis of microRNA …
pathway. In this canonical role, Dicer is a critical regulator of the biogenesis of microRNA …
Long noncoding RNAs: functional surprises from the RNA world
Most of the eukaryotic genome is transcribed, yielding a complex network of transcripts that
includes tens of thousands of long noncoding RNAs with little or no protein-coding capacity …
includes tens of thousands of long noncoding RNAs with little or no protein-coding capacity …
Biogenesis of small RNAs in animals
Small RNAs of 20–30 nucleotides can target both chromatin and transcripts, and thereby
keep both the genome and the transcriptome under extensive surveillance. Recent progress …
keep both the genome and the transcriptome under extensive surveillance. Recent progress …
A genome-scale shRNA resource for transgenic RNAi in Drosophila
Existing transgenic RNAi resources in Drosophila melanogaster based on long double-
stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in …
stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in …
Small silencing RNAs: an expanding universe
Since the discovery in 1993 of the first small silencing RNA, a dizzying number of small RNA
classes have been identified, including microRNAs (miRNAs), small interfering RNAs …
classes have been identified, including microRNAs (miRNAs), small interfering RNAs …