A decade of advances in transposon-insertion sequencing
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …
methods, which combine genome-wide transposon mutagenesis with high-throughput …
Activity-based annotation: the emergence of systems biochemistry
KY Rhee, RS Jansen, C Grundner - Trends in biochemical sciences, 2022 - cell.com
Current tools to annotate protein function have failed to keep pace with the speed of DNA
sequencing and exponentially growing number of proteins of unknown function (PUFs). A …
sequencing and exponentially growing number of proteins of unknown function (PUFs). A …
Loss of YhcB results in dysregulation of coordinated peptidoglycan, LPS and phospholipid synthesis during Escherichia coli cell growth
The cell envelope is essential for viability in all domains of life. It retains enzymes and
substrates within a confined space while providing a protective barrier to the external …
substrates within a confined space while providing a protective barrier to the external …
High-throughput interaction screens illuminate the role of c-di-AMP in cyanobacterial nighttime survival
The broadly conserved signaling nucleotide cyclic di-adenosine monophosphate (c-di-AMP)
is essential for viability in most bacteria where it has been studied. However …
is essential for viability in most bacteria where it has been studied. However …
Genome‐Wide CRISPRi Screening of Key Genes for Recombinant Protein Expression in Bacillus Subtilis
X Zhu, H Luo, X Yu, H Lv, L Su, K Zhang… - Advanced …, 2024 - Wiley Online Library
Bacillus subtilis is an industrially important microorganism that is often used as a microbial
cell factory for the production of recombinant proteins due to its food safety, rapid growth …
cell factory for the production of recombinant proteins due to its food safety, rapid growth …
When Dicty met Myco, a (not so) romantic story about one amoeba and its intracellular pathogen
E Cardenal-Munoz, C Barisch, LH Lefrancois… - Frontiers in cellular …, 2018 - frontiersin.org
In recent years, Dictyostelium discoideum has become an important model organism to
study the cell biology of professional phagocytes. This amoeba not only shares many …
study the cell biology of professional phagocytes. This amoeba not only shares many …
Gene fitness of Azotobacter vinelandii under diazotrophic growth
Azotobacter vinelandii is a nitrogen-fixing free-living soil microbe that has been studied for
decades in relation to biological nitrogen fixation (BNF). It is highly amenable to genetic …
decades in relation to biological nitrogen fixation (BNF). It is highly amenable to genetic …
Transposon sequencing: A powerful tool for the functional genomic study of food-borne pathogens
Background Genome-wide functional genetic studies of food-borne pathogens could
provide a comprehensive understanding of the mechanisms underline their survival and …
provide a comprehensive understanding of the mechanisms underline their survival and …
A guide to Mycobacterium mutagenesis
K Borgers, K Vandewalle, N Festjens… - The FEBS …, 2019 - Wiley Online Library
The genus Mycobacterium includes several pathogens that cause severe disease in
humans, like Mycobacterium tuberculosis (M. tb), the infectious agent causing tuberculosis …
humans, like Mycobacterium tuberculosis (M. tb), the infectious agent causing tuberculosis …
Multiplexed strain phenoty** defines consequences of genetic diversity in Mycobacterium tuberculosis for infection and vaccination outcomes
There is growing evidence that genetic diversity in Mycobacterium tuberculosis, the
causative agent of tuberculosis, contributes to the outcomes of infection and public health …
causative agent of tuberculosis, contributes to the outcomes of infection and public health …