Recombinant protein expression in Escherichia coli: advances and challenges

GL Rosano, EA Ceccarelli - Frontiers in microbiology, 2014 - frontiersin.org
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications

A Bhatwa, W Wang, YI Hassan, N Abraham… - … in Bioengineering and …, 2021 - frontiersin.org
Recombinant proteins are becoming increasingly important for industrial applications, where
Escherichia coli is the most widely used bacterial host for their production. However, the …

Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements

J Kaur, A Kumar, J Kaur - International journal of biological …, 2018 - Elsevier
E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …

Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli

HP Sørensen, KK Mortensen - Microbial cell factories, 2005 - Springer
Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural
protein sources rarely meet the requirements for quantity, ease of isolation or price and …

Ecology of aspergillosis: insights into the pathogenic potency of Aspergillus fumigatus and some other Aspergillus species

C Paulussen, JE Hallsworth… - Microbial …, 2017 - Wiley Online Library
Fungi of the genus Aspergillus are widespread in the environment. Some Aspergillus
species, most commonly Aspergillus fumigatus, may lead to a variety of allergic reactions …

Psychrophilic lifestyles: mechanisms of adaptation and biotechnological tools

T Collins, R Margesin - Applied microbiology and biotechnology, 2019 - Springer
Cold-adapted microorganisms inhabiting permanently low-temperature environments were
initially just a biological curiosity but have emerged as rich sources of numerous valuable …

Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies

S Sahdev, SK Khattar, KS Saini - Molecular and cellular biochemistry, 2008 - Springer
Among the various expression systems employed for the over-production of proteins,
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …

Missense meanderings in sequence space: a biophysical view of protein evolution

MA DePristo, DM Weinreich, DL Hartl - Nature Reviews Genetics, 2005 - nature.com
Proteins are finicky molecules; they are barely stable and are prone to aggregate, but they
must function in a crowded environment that is full of degradative enzymes bent on their …

A new analysis of Mars “special regions”: findings of the second MEPAG Special Regions Science Analysis Group (SR-SAG2)

JD Rummel, DW Beaty, MA Jones, C Bakermans… - 2014 - liebertpub.com
A committee of the Mars Exploration Program Analysis Group (MEPAG) has reviewed and
updated the description of Special Regions on Mars as places where terrestrial organisms …

Discovery, molecular mechanisms, and industrial applications of cold-active enzymes

M Santiago, CA Ramírez-Sarmiento… - Frontiers in …, 2016 - frontiersin.org
Cold-active enzymes constitute an attractive resource for biotechnological applications.
Their high catalytic activity at temperatures below 25° C makes them excellent biocatalysts …