Metagenomics-enabled microbial surveillance

KKK Ko, KR Chng, N Nagarajan - Nature Microbiology, 2022 - nature.com
Lessons learnt from the COVID-19 pandemic include increased awareness of the potential
for zoonoses and emerging infectious diseases that can adversely affect human health …

Advances in chemical and biological methods to identify microorganisms—from past to present

R Franco-Duarte, L Černáková, S Kadam, K S. Kaushik… - Microorganisms, 2019 - mdpi.com
Fast detection and identification of microorganisms is a challenging and significant feature
from industry to medicine. Standard approaches are known to be very time-consuming and …

RESCRIPt: Reproducible sequence taxonomy reference database management

MS Robeson, DR O'Rourke, BD Kaehler… - PLoS computational …, 2021 - journals.plos.org
Nucleotide sequence and taxonomy reference databases are critical resources for
widespread applications including marker-gene and metagenome sequencing for …

Comparison between 16S rRNA and shotgun sequencing data for the taxonomic characterization of the gut microbiota

F Durazzi, C Sala, G Castellani, G Manfreda… - Scientific reports, 2021 - nature.com
In this paper we compared taxonomic results obtained by metataxonomics (16S rRNA gene
sequencing) and metagenomics (whole shotgun metagenomic sequencing) to investigate …

Robust taxonomic classification of uncharted microbial sequences and bins with CAT and BAT

FAB Von Meijenfeldt, K Arkhipova, DD Cambuy… - Genome biology, 2019 - Springer
Current-day metagenomics analyses increasingly involve de novo taxonomic classification
of long DNA sequences and metagenome-assembled genomes. Here, we show that the …

Advances in metagenomics and its application in environmental microorganisms

L Zhang, FX Chen, Z Zeng, M Xu, F Sun… - Frontiers in …, 2021 - frontiersin.org
Metagenomics is a new approach to study microorganisms obtained from a specific
environment by functional gene screening or sequencing analysis. Metagenomics studies …

KrakenUniq: confident and fast metagenomics classification using unique k-mer counts

FP Breitwieser, DN Baker, SL Salzberg - Genome biology, 2018 - Springer
False-positive identifications are a significant problem in metagenomics classification. We
present KrakenUniq, a novel metagenomics classifier that combines the fast k-mer-based …

Metagenomic approaches in microbial ecology: an update on whole-genome and marker gene sequencing analyses

AE Pérez-Cobas, L Gomez-Valero… - Microbial …, 2020 - microbiologyresearch.org
Metagenomics and marker gene approaches, coupled with high-throughput sequencing
technologies, have revolutionized the field of microbial ecology. Metagenomics is a culture …

Metagenome analysis using the Kraken software suite

J Lu, N Rincon, DE Wood, FP Breitwieser… - Nature protocols, 2022 - nature.com
Metagenomic experiments expose the wide range of microscopic organisms in any
microbial environment through high-throughput DNA sequencing. The computational …

[HTML][HTML] Metagenomics next-generation sequencing tests take the stage in the diagnosis of lower respiratory tract infections

Z Diao, D Han, R Zhang, J Li - Journal of advanced research, 2022 - Elsevier
Background Metagenomic next-generation sequencing (mNGS) has changed the diagnosis
landscape of lower respiratory tract infections (LRIs). With the development of newer …