An integrated technology for quantitative wide mutational scanning of human antibody Fab libraries

BM Petersen, MB Kirby, KM Chrispens, OM Irvin… - Nature …, 2024 - nature.com
Antibodies are engineerable quantities in medicine. Learning antibody molecular
recognition would enable the in silico design of high affinity binders against nearly any …

Progress in application of cyclic single-stranded nucleic acids

X Liu, J Tong, M Li, L Li, W Cai, L Wang, M Sun - Journal of Biotechnology, 2024 - Elsevier
Cyclic nucleic acids are biologically stable against nucleic acid exonucleases due to the
absence of 5'and 3'termini. Studies of cyclic nucleic acids mainly focus on cyclic single …

Standardizing cassette‐based deep mutagenesis by Golden Gate assembly

N Daffern, IM Francino‐Urdaniz… - Biotechnology and …, 2024 - Wiley Online Library
Protocols for the construction of large, deeply mutagenized protein encoding libraries via
Golden Gate assembly of synthetic DNA cassettes employ disparate, system‐specific …

Benchmarking cassette-based deep mutagenesis by Golden Gate assembly

N Daffern, I Francino-Urdaniz, ZT Baumer… - bioRxiv, 2023 - biorxiv.org
Protocols for the construction of large, deeply mutagenized protein encoding libraries via
Golden Gate assembly of synthetic DNA cassettes employ disparate, system specific …

Commonality in Natural Antibody Fitness Landscapes

BM Petersen - 2024 - search.proquest.com
Despite the success of current vaccine technologies, hypervariable viruses such as SARS-
CoV-2 and influenza pose a significant threat to public health. To address this problem …

Dynamic Regulation of Engineered T7 RNA Polymerases

ZT Baumer - 2024 - search.proquest.com
Proteins (alongside carbohydrates, nucleic acids, and lipids) are one of the main
macromolecules of cellular biology, persisting in all areas of life. Arguably the most exciting …