[HTML][HTML] DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work

H Weigand, AJ Beermann, F Čiampor, FO Costa… - Science of the Total …, 2019 - Elsevier
Effective identification of species using short DNA fragments (DNA barcoding and DNA
metabarcoding) requires reliable sequence reference libraries of known taxa. Both …

Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

K Deiner, HM Bik, E Mächler, M Seymour… - Molecular …, 2017 - Wiley Online Library
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive

D Hering, A Borja, JI Jones, D Pont, P Boets… - Water research, 2018 - Elsevier
Assessment of ecological status for the European Water Framework Directive (WFD) is
based on “Biological Quality Elements”(BQEs), namely phytoplankton, benthic flora, benthic …

Methodology of fish eDNA and its applications in ecology and environment

S Wang, Z Yan, B Hänfling, X Zheng, P Wang… - Science of the Total …, 2021 - Elsevier
Fish environmental DNA (eDNA) studies have made substantial progress during the past
decade, and significant advances in monitoring fishes have been gained by taking …

Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap

T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …

Assessing strengths and weaknesses of DNA metabarcoding‐based macroinvertebrate identification for routine stream monitoring

V Elbrecht, EE Vamos, K Meissner… - Methods in Ecology …, 2017 - Wiley Online Library
DNA metabarcoding holds great promise for the assessment of macroinvertebrates in
stream ecosystems. However, few large‐scale studies have compared the performance of …

A validation scale to determine the readiness of environmental DNA assays for routine species monitoring

B Thalinger, K Deiner, LR Harper, HC Rees… - Environmental …, 2021 - Wiley Online Library
The use of environmental DNA (eDNA) analysis for species monitoring requires rigorous
validation—from field sampling to the analysis of PCR‐based results—for meaningful …

Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds

LR Harper, AS Buxton, HC Rees, K Bruce, R Brys… - Hydrobiologia, 2019 - Springer
Environmental DNA (eDNA) analysis is a rapid, non-invasive, cost-efficient biodiversity
monitoring tool with enormous potential to inform aquatic conservation and management …