16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls

JM Janda, SL Abbott - Journal of clinical microbiology, 2007 - Am Soc Microbiol
The use of 16S rRNA gene sequences to study bacterial phylogeny and taxonomy has been
by far the most common housekee** genetic marker used for a number of reasons. These …

Performance and application of 16S rRNA gene cycle sequencing for routine identification of bacteria in the clinical microbiology laboratory

DL Church, L Cerutti, A Gürtler, T Griener… - Clinical microbiology …, 2020 - Am Soc Microbiol
This review provides a state-of-the-art description of the performance of Sanger cycle
sequencing of the 16S rRNA gene for routine identification of bacteria in the clinical …

[HTML][HTML] Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories

PCY Woo, SKP Lau, JLL Teng, H Tse… - Clinical Microbiology and …, 2008 - Elsevier
In the last decade, as a result of the widespread use of PCR and DNA sequencing, 16S
rDNA sequencing has played a pivotal role in the accurate identification of bacterial isolates …

Role of coagulase-negative staphylococci in human disease

A Piette, G Verschraegen - Veterinary microbiology, 2009 - Elsevier
Coagulase-negative staphylococci (CNS) are normal inhabitants of human skin and mucous
membranes. They have long been dismissed as culture contaminants, but now the …

Species identification by MALDI-TOF MS and gap PCR–RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and …

NM Rosa, M Penati, S Fusar-Poli, MF Addis, S Tola - Veterinary Research, 2022 - Springer
Staphylococci and streptococci are common causes of intramammary infection in small
ruminants, and reliable species identification is crucial for understanding epidemiology and …

Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP

S Taponen, H Simojoki, M Haveri, HD Larsen… - Veterinary …, 2006 - Elsevier
The coagulase-negative staphylococcal species causing mastitis in lactating cattle were
identified and possible differences in the clinical characteristics or persistence of mastitis …

Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology

CD Sibley, G Peirano, DL Church - Infection, Genetics and Evolution, 2012 - Elsevier
Clinical microbiology laboratories worldwide have historically relied on phenotypic methods
(ie, culture and biochemical tests) for detection, identification and characterization of …

Identification, ty**, ecology and epidemiology of coagulase negative staphylococci associated with ruminants

W Vanderhaeghen, S Piepers, F Leroy… - The Veterinary …, 2015 - Elsevier
Since phenotypic methods to identify coagulase negative staphylococci (CNS) from the milk
of ruminants often yield unreliable results, methods for molecular identification based on …

Staphylococcus Epidermidis as a Cause of Bacteremia

S Kleinschmidt, F Huygens, J Faoagali… - Future …, 2015 - Taylor & Francis
Staphylococcus epidermidis is a biofilm-producing commensal organism found ubiquitously
on human skin and mucous membranes, as well as on animals and in the environment …

First Case of Staphylococcus pseudintermedius Infection in a Human

L Van Hoovels, A Vankeerberghen, A Boel… - Journal of clinical …, 2006 - Am Soc Microbiol
We present the first clinical report of a Staphylococcus pseudintermedius infection in a
human. Biochemically, S. pseudintermedius can be easily misidentified as S. aureus …