Mass spectrometry‐based protein–protein interaction networks for the study of human diseases
A better understanding of the molecular mechanisms underlying disease is key for
expediting the development of novel therapeutic interventions. Disease mechanisms are …
expediting the development of novel therapeutic interventions. Disease mechanisms are …
Subcellular proteomics
The eukaryotic cell is compartmentalized into subcellular niches, including membrane-
bound and membrane-less organelles. Proteins localize to these niches to fulfil their …
bound and membrane-less organelles. Proteins localize to these niches to fulfil their …
OpenCell: Endogenous tagging for the cartography of human cellular organization
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era.
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …
[HTML][HTML] Dual proteome-scale networks reveal cell-specific remodeling of the human interactome
Thousands of interactions assemble proteins into modules that impart spatial and functional
organization to the cellular proteome. Through affinity-purification mass spectrometry, we …
organization to the cellular proteome. Through affinity-purification mass spectrometry, we …
Robust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC–MS/MS
Nano-flow liquid chromatography tandem mass spectrometry (nano-flow LC–MS/MS) is the
mainstay in proteome research because of its excellent sensitivity but often comes at the …
mainstay in proteome research because of its excellent sensitivity but often comes at the …
The SARS-CoV-2 RNA Interactome
SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its abilities to
repurpose host RNA-binding proteins (RBPs) and to evade antiviral RBPs. To uncover the …
repurpose host RNA-binding proteins (RBPs) and to evade antiviral RBPs. To uncover the …
A high-density human mitochondrial proximity interaction network
H Antonicka, ZY Lin, A Janer, MJ Aaltonen… - Cell metabolism, 2020 - cell.com
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from
all mitochondrial sub-compartments, to create a high-resolution human mitochondrial …
all mitochondrial sub-compartments, to create a high-resolution human mitochondrial …
Combined proximity labeling and affinity purification− mass spectrometry workflow for map** and visualizing protein interaction networks
X Liu, K Salokas, RG Weldatsadik, L Gawriyski… - Nature protocols, 2020 - nature.com
Affinity purification coupled with mass spectrometry (AP–MS) and proximity-dependent
biotinylation identification (BioID) methods have made substantial contributions to …
biotinylation identification (BioID) methods have made substantial contributions to …
FFAT motif phosphorylation controls formation and lipid transfer function of inter‐organelle contacts
T Di Mattia, A Martinet, S Ikhlef, AG McEwen… - The EMBO …, 2020 - embopress.org
Organelles are physically connected by membrane contact sites. The endoplasmic reticulum
possesses three major receptors, VAP‐A, VAP‐B, and MOSPD2, which interact with proteins …
possesses three major receptors, VAP‐A, VAP‐B, and MOSPD2, which interact with proteins …
Predicting protein condensate formation using machine learning
Membraneless organelles are liquid condensates, which form through liquid-liquid phase
separation. Recent advances show that phase separation is essential for cellular …
separation. Recent advances show that phase separation is essential for cellular …