Covering: 2005 up to 2019 Natural products are of paramount importance in human
medicine. Not only are most antibacterial and anticancer drugs derived directly from or …

Phage integrases are enzymes that mediate unidirectional site-specific recombination
between two DNA recognition sequences, the phage attachment site, attP, and the bacterial …

Germ-line transformation via transposable elements is a powerful tool to study gene function
in Drosophila melanogaster. However, some inherent characteristics of transposon …

INTRODUCTION Living systems execute regulatory programs and exhibit specific
phenotypes depending on the identity and timing of chemical signals, but general strategies …

The φC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and
mammalian cells, mediating unidirectional site-specific recombination between its attB and …

We describe a transgenesis platform for Drosophila melanogaster that integrates three
recently developed technologies: a conditionally amplifiable bacterial artificial chromosome …

The loss of transgene expression has been a major obstacle to the development of nonviral
vectors for the treatment of human diseases. We previously demonstrated that bacterial DNA …

The development of two-component expression systems in Drosophila melanogaster, one of
the most powerful genetic models, has allowed the precise manipulation of gene function in …

Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues.
To improve minicircle production, we genetically modified Escherichia coli to construct a …

It has been assumed that DNA synthesis by the leading-and lagging-strand polymerases in
the replisome must be coordinated to avoid the formation of significant gaps in the nascent …