Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used
mammalian host for large-scale commercial production of therapeutic proteins. Recent …

From 2006 to 2011, an average of 15 novel recombinant protein therapeutics have been
approved by US Food and Drug Administration (FDA) annually. In addition, the expiration of …

Almost all of the 200 or so approved biopharmaceuticals have been produced in one of
three host systems: the bacterium Escherichia coli, yeasts (Saccharomyces cerevisiae …

Metabolomics aims to quantify all metabolites within an organism, thereby providing
valuable insight into the metabolism of cells. To study intracellular metabolites, they are first …

Although the cold-shock response has now been studied in a number of different organisms
for several decades, it is only in the last few years that we have begun to understand the …

The commercial production of monoclonal antibodies (mAbs) has revolutionized the
treatment of many diseases, including cancer, multiple sclerosis, and rheumatoid arthritis …

Chinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial
production of biopharmaceutical products, such as monoclonal antibodies, human growth …

Host cell proteins (HCPs) constitute a major group of impurities for biologic drugs produced
using cell culture technology. HCPs are required to be closely monitored and adequately …

Effective development of host cells for therapeutic protein production is hampered by the
poor characterization of cellular transfection. Here, we employed a multi-omics-based …

The application of mild hypothermic conditions to cell culture is a routine industrial practice
used to improve recombinant protein production. However, a thorough understanding of the …