Quantification of viable granulosa cells in murine ovarian follicles

M Asaduzzaman, RJ Rodgers… - Biotechnic & …, 2020 - Taylor & Francis
Ovarian follicle growth and oocyte maturation depend on the viability of granulosa cells
(GC). We quantified GC in whole mouse follicles. Single follicles were isolated from adult …

Three dimensional in vitro culture of murine secondary follicles in a defined synthetic matrix

M Asaduzzaman, X Cui… - … of Biomaterials and …, 2018 - researchnow.flinders.edu.au
Ovarian follicle growth in three dimensional (3D) matrices in vitro has limitations: a) matrices
don't expand as follicles grow, b) requirements for enzyme-mediated retrieval, and c) animal …

Establishment of a protocol for the isolation of ovarian preantral follicles derived from collared peccaries (Pecari tajacu)

LB Campos, AM da Silva, SSJ Moreira, CS Santos… - Zygote, 2019 - cambridge.org
We compare the efficiency of mechanical or enzymatic methods, and their combination, for
the isolation of ovarian preantral follicles (PFs) from collared peccaries. The ovaries from six …

Interaction between oocytes, cortical germ cells and granulosa cells of the mouse and bat, following the dissociation–re-aggregation of adult ovaries

TJ Porras-Gómez, N Moreno-Mendoza - Zygote, 2020 - cambridge.org
It is widely accepted that the oocyte plays a very active role in promoting the growth of the
follicle by directing the differentiation of granulosa cells and secreting paracrine growth …

Ovarian follicle disaggregation to assess granulosa cell viability

M Asaduzzaman, DF Gonzalez… - … Journal of Clinical …, 2018 - researchnow.flinders.edu.au
Background: Mammalian ovaries contain follicles containing an oocyte enclosed by layers of
granulosa cells (GC). Follicle growth and oocyte maturation are largely dependent on GC …

[PDF][PDF] Role of chemotherapeutics in ovarian failure

M Asaduzzaman - 2018 - flex.flinders.edu.au
The treatment of cancers in adolescent and young adult (AYA) women can cause premature
ovarian failure and infertility (Ben-Aharon et al. 2010a; Knight & Glister 2006; Meirow et al …

[PDF][PDF] Capítulo IV-Establishment of an effective protocol for the isolation of ovarian preantral follicles derived from collared peccaries (Pecari tajacu)

LB Campos, AM da Silva… - … PRÉ-ANTRAIS DE …, 2019 - repositorio.ufersa.edu.br
We compare the efficiency of mechanical and enzymatic methods alone or combination for
the isolation of ovarian preantral follicles (PFs) from collared peccaries. The ovaries from 6 …