More than 10 years ago, we published the paper describing the mothur software package in
Applied and Environmental Microbiology. Our goal was to create a comprehensive package …

The profound influence of microorganisms on human life and global biogeochemical cycles
underlines the value of studying the biogeography of microorganisms, exploring microbial …

Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for
investigating microbial populations. In such experiments, sequence errors caused by PCR …

DNA metabarcoding is promising for cost-effective biodiversity monitoring, but reliable
diversity estimates are difficult to achieve and validate. Here we present and validate a …

Abstract Motivation The 16S ribosomal RNA (rRNA) gene is widely used to survey microbial
communities. Sequences are often clustered into Operational Taxonomic Units (OTUs) as …

Nonalcoholic fatty liver disease (NAFLD) including nonalcoholic steatohepatitis (NASH) has
reached epidemic proportions with no pharmacological therapy approved. Lower circulating …

Motivation: Next-generation sequencing produces vast amounts of data with errors that are
difficult to distinguish from true biological variation when coverage is low. Results: We …

Low-input agricultural systems aim at reducing the use of synthetic fertilizers and pesticides
in order to improve sustainable production and ecosystem health. Despite the integral role of …

Background The plant microbiome represents one of the key determinants of plant health
and productivity by providing a plethora of functional capacities such as access to low …

Amplified marker-gene sequences can be used to understand microbial community
structure, but they suffer from a high level of sequencing and amplification artifacts. The …