The de novo construction of a living organism is a compelling vision. Despite the astonishing
technologies developed to modify living cells, building a functioning cell “from scratch” has …

Naturally evolved organisms typically have large genomes that enable their survival and
growth under various conditions. However, the complexity of genomes often precludes our …

Advances in systems and synthetic biology have propelled the construction of reduced
bacterial genomes. Genome reduction was initially focused on exploring properties of …

Mycoplasmas are atypical bacteria with small genomes that necessitate colonization of their
respective animal or plant hosts as obligate parasites, whether as pathogens, or …

Synthetic genomics seeks to design and construct entire genomes to mechanistically dissect
fundamental questions of genome function and to engineer organisms for diverse …

Fast growth phenotypes are achieved through optimal transcriptomic allocation, in which
cells must balance tradeoffs in resource allocation between diverse functions. One such …

Introduction Mycoplasma hominis (M. hominis) belongs to the class Mollicutes,
characterized by a very small genome size, reduction of metabolic pathways, including …

Mesoplasma florum is an emerging model organism for systems and synthetic biology due
to its small genome (∼ 800 kb) and fast growth rate. While M. florum was isolated and first …

Mycoplasmas have been widely investigated for their pathogenicity, as well as for genomics
and synthetic biology. Conventionally, transformation of mycoplasmas was not highly …

After decades of development, DNA synthesis, assembly, and sequencing technologies
have reached a high level, allowing faster and cheaper acquirements of synthetic genes or …