Synthetic DNA is of increasing demand across many sectors of research and commercial
activities. Engineering biology, therapy, data storage and nanotechnology are set for rapid …

Forward genetic screens are powerful tools for the discovery and functional annotation of
genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short …

Acquired resistance to tyrosine kinase inhibitors (TKI), such as osimertinib used to treat
EGFR-mutant lung adenocarcinomas, limits long-term efficacy and is frequently caused by …

De novo protein design holds promise for creating small stable proteins with shapes
customized to bind therapeutic targets. We describe a massively parallel approach for …

The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes,
circuits, and even entire genomes by gene synthesis methods has become an enabling …

The TP53 gene is frequently mutated in human cancer. Research has focused
predominantly on six major" hotspot" codons, which account for only∼ 30% of cancer …

For over 60 years, the synthetic production of new DNA sequences has helped researchers
understand and engineer biology. Here we summarize methods and caveats for the de novo …

Existing transgenic RNAi resources in Drosophila melanogaster based on long double-
stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in …

Despite extensive research, our understanding of the rules according to which cis-regulatory
sequences are converted into gene expression is limited. We devised a method for …

INTRODUCTION The recruitment of the ribosome to a specific mRNA is a critical step in the
production of proteins in cells. In addition to a general recognition of the “cap” structure at …